Chilling slows anaerobic metabolism to improve anoxia tolerance of insects
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Insects are renowned for their ability to survive anoxia. Anoxia tolerance may be enhanced during chilling through metabolic suppression.
Here, the metabolomic response of insects to anoxia, both with and without chilling, for different durations (12–36 h) was examined to assess the potential cross-tolerance mechanisms.
Chilling during anoxia (cold anoxia) significantly improved survival relative to anoxia at warmer temperatures. Reduced intermediate metabolites and increased lactic acid, indicating a switch to anaerobic metabolism, were characteristic of larvae in anoxia.
Anoxia tolerance was correlated survival improvements after cold anoxia were correlated with a reduction in anaerobic metabolism.
KeywordsAnoxia Hypoxia Cold Lactic acid False codling moth
XSIT kindly provided larvae. We are grateful for comments by anonymous referees that helped improve the work.
This research was completed with financial support from International Atomic Energy Agency (CRP), Hortgro Stellenbosch and Citrus Research International to JST and metabolomics analysis was supported by the Czech Science Foundation, No. 13-18509S to PS. LB was supported by National Research Foundation (NRF) DST Postdoctoral fellowship, JGS was supported by a Sapere Aude DFF-Starting grant from The Danish Council for Independent Research| Natural Sciences and JST was supported by NRF Incentive Funding and Sub-Committee B (Stellenbosch University).
Compliance with ethical standards
Conflict of interest
L Boardman, J. Sørensen, V. Kostal, P. Simek and J. Terblanche have no conflict of interest to declare.
All applicable international, national and/or institutional guidelines for the care and use of animals were followed.
Informed consent and ethical approval was not required for this study.
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