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Metabolomics

, Volume 10, Issue 5, pp 805–815 | Cite as

Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis–mass spectrometry

  • Jieyu Zhao
  • Chunxiu Hu
  • Jun Zeng
  • Yanni Zhao
  • Junjie Zhang
  • Yuwei Chang
  • Lili Li
  • Chunxia Zhao
  • Xin Lu
  • Guowang XuEmail author
Original Article

Abstract

Many metabolites in plant are highly polar and ionic. Their analysis using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry can be problematic. Therefore a capillary electrophoresis–mass spectrometry (CE–MS) method with charge-driven separation characteristic was developed to investigate polar metabolites in tobacco. To obtain as many features as possible, extraction of polar metabolites was optimized by the design of experiments and evaluated by univariate statistics. Method validation was carried out to evaluate the analytical characteristics including calibration curve, precision, sample stability and extraction reproducibility. The developed method was successfully applied in studying 30 tobacco leaves obtained from Yunnan and Guizhou provinces in China. A total of 154 polar metabolites were identified based on available database. Multivariate pattern recognition clearly revealed the metabolic differences between the two geographic areas and 43 significantly different metabolites were defined by the non-parametric hypothesis test (Mann–Whitney U test) and false discovery rate. Some key metabolites involved in photosynthesis such as ribulose 1,5-disphosphate, fructose 1,6-diphosphate, glycine, betaine, GABA and serine were found to be susceptible to environmental conditions. This study shows that the metabolic profiling based on CE–MS can clearly discriminate tobacco leaves of different geographical origins and understand the relationship between plant metabolites and their geographical origins.

Keywords

Capillary electrophoresis Mass spectrometry Plant metabolomics Method validation Tobacco leaf 

Supplementary material

11306_2014_631_MOESM1_ESM.docx (738 kb)
Supplementary material 1 (DOCX 738 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jieyu Zhao
    • 1
  • Chunxiu Hu
    • 1
  • Jun Zeng
    • 1
  • Yanni Zhao
    • 1
  • Junjie Zhang
    • 1
  • Yuwei Chang
    • 1
  • Lili Li
    • 1
  • Chunxia Zhao
    • 1
  • Xin Lu
    • 1
  • Guowang Xu
    • 1
    Email author
  1. 1.Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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