Metabolomics

, Volume 9, Issue 4, pp 919–930 | Cite as

Metabolite profiling reveals tissue- and temperature-specific metabolomic responses in thermoregulatory male florets of Dracunculus vulgaris (Araceae)

  • Kikukatsu Ito
  • Hideyuki Takahashi
  • Yui Umekawa
  • Tomohiro Imamura
  • Shuji Kawasaki
  • Takafumi Ogata
  • Yusuke Kakizaki
  • Roger S. Seymour
Original Article

Abstract

The male part of the spadix of Dracunculus vulgaris exhibits a degree of temperature regulation by inversely controlled heat production over a 20–35 °C range of tissue temperature. To clarify the effects of temperature on cellular metabolism, comparative analysis was performed using 51 metabolites from two distinct tissues (florets and pith) of thermogenic male spadices that had been temperature clamped at either 20 (to produce high respiration) or 35 °C (to produce low respiration). Principal component analysis and hierarchical clustering analysis showed that changes in metabolites in the florets, but not in the pith, were associated with temperature change. The energy charge in the florets treated at 20 °C was significantly higher than that of the florets treated at 35 °C. This indicated the presence of an increased energy-producing pathway that ultimately led to an increased level of thermogenesis at 20 °C. Intriguingly, succinate, a direct substrate for complex II in the mitochondrial respiratory chain, was the metabolite most significantly affected in our analysis, with its concentration in the florets 3.5 times higher at 20 than at 35 °C. However, the mitochondria fed with succinate showed that state 2 and 3 respirations and the capacity of the alternative and cytochrome pathways were all significantly higher at 35 than at 20 °C. Taken together, the results show that the male florets are the primary sites for temperature-induced changes in metabolomic pathways, although succinate-stimulated mitochondrial respiration, per sé, is not the control mechanism for thermoregulation in D. vulgaris.

Keywords

Dracunculusvulgaris Thermoregulation Temperature Hierarchical clustering analysis Principal component analysis Energy charge 

Supplementary material

11306_2013_509_MOESM1_ESM.pptx (75 kb)
Supplementary material 1 (PPTX 74 kb)
11306_2013_509_MOESM2_ESM.pptx (783 kb)
Supplementary material 2 (PPTX 783 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Kikukatsu Ito
    • 1
  • Hideyuki Takahashi
    • 2
  • Yui Umekawa
    • 1
  • Tomohiro Imamura
    • 2
  • Shuji Kawasaki
    • 3
  • Takafumi Ogata
    • 1
    • 4
  • Yusuke Kakizaki
    • 5
  • Roger S. Seymour
    • 6
  1. 1.Cryobiofrontier Research Center, Faculty of AgricultureIwate UniversityMoriokaJapan
  2. 2.Iwate Biotechnology Research CenterKitakamiJapan
  3. 3.Faculty of Humanities and Social SciencesIwate UniversityMoriokaJapan
  4. 4.Aoba Kasei Co. Ltd.SendaiJapan
  5. 5.United Graduate School of Agricultural Sciences, Iwate UniversityMoriokaJapan
  6. 6.Ecology and Evolutional Biology, University of AdelaideAdelaideAustralia

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