Application of direct analysis in real time ionization–mass spectrometry (DART–MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud
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Metabolomic fingerprinting enabled by ambient mass spectrometry employing a direct analysis in real time (DART) ion source coupled to a medium–high resolution/accurate mass time-of-flight mass spectrometer (TOFMS) was used as a tool for differentiation between chickens fed by feed that contained 5–8 % (w/w) of chicken bone meal (a banned component) and those representing a reference group, i.e. grown otherwise under the same conditions. In the first step, the sample extraction and DART–TOFMS instrumental conditions were optimized to obtain the broadest possible representation of ionizable compounds occurring in the extracts obtained from chicken muscle and feed on which experimental animals were grown. To this end, a simultaneous (all-in-one) extraction procedure was developed employing water and cyclohexane mixture as the extraction solvents. Under these conditions both polar as well as non-polar metabolites were isolated within a single extraction step. In the next step, metabolomic fingerprints of a large set of chicken muscle and feed extracts were acquired. In the final phase, the experimental data were statistically evaluated using principal component analysis and orthogonal partial least squares discriminant analysis. In general, differentiation of chicken muscle according to diet (feed with and without the addition of chicken bone meal) was feasible. Additional experiments conducted after 6 months confirmed applicability of this approach. Correct classification was obtained based on the assessment of polar as well as non-polar extracts fingerprints. However, the analysis of non-polar extracts showed that the pattern of triacylglycerols is more prone to seasonal variability and/or type of raw materials used during feed preparation which obscures the bone meal impact to some extent.
KeywordsDirect analysis in real time (DART) Mass spectrometry Chicken Feed Authenticity Metabolomic fingerprinting
The financial support of the Ministry of Education, Youth and Sports of the Czech Republic (Projects MSM 6046137305, MSMT No. 21/2012) and the Ministry of Agriculture of the Czech Republic (NAZV-QI91B306) is acknowledged. The authors thank to Zdenek Jandejsek from Rabbit CZ, a.s. for the preparation of feeds and the breeding of chickens.
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- Bai, Y., Zhang, J., Bai, Y., & Liu, H. (2012). Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice. Analytical and Bioanalytical Chemistry, 403, 2307–2314.PubMedCrossRefGoogle Scholar
- Cantor, A. H., Decker, E. A., & Collins, V. P. (2008). Fatty acids in poultry and egg products. In C. K. Chow (Ed.), Fatty acids in foods and their health implications (pp. 127–154). Boca Raton, FL: CRC Press.Google Scholar
- Danhelova, H., Hradecky, J., Prinosilova, S., Cajka, T., Riddellova, K., Vaclavik, L., et al. (2012). Rapid analysis of caffeine in various coffee samples employing direct analysis in real time ionization–high-resolution mass spectrometry. Analytical and Bioanalytical Chemistry, 403, 2883–2889.PubMedCrossRefGoogle Scholar
- French, P., Stanton, C., Lawless, F., O’Riordan, E. G., Monahan, F. J., Caffrey, P. J., et al. (2000). Fatty acid composition, including conjugated linoleic acid, of intramuscular fat from steers offered grazed grass, grass silage, or concentrate-based diets. Journal of Animal Science, 78, 2849–2855.PubMedGoogle Scholar
- Gu, H., Pan, Z., Xi, B., Asiago, V., Musselman, B., & Raftery, D. (2011). Principal component directed partial least squares analysis for combining nuclear magnetic resonance and mass spectrometry data in metabolomics: Application to the detection of breast cancer. Analytica Chimica Acta, 686, 57–63.PubMedCrossRefGoogle Scholar
- JinGe, D., XiaoTing, Z., JiaCheng, H., YunFang, Q., & Ke, S. (2009). Effects of dietary supplementation of glutamine on meat quality and antioxidant indexes of broilers. Chinese Journal of Animal Nutrition, 21, 245–250.Google Scholar
- Regulation (EC) No 1774/2002 of The European Parliament and of The Council of 3 October 2002 laying down health rules concerning animal by-products not intended for human consumption. http://eur-lex.europa.eu/LexUriServ/site/en/consleg/2002/R/02002R1774-20060401-en.pdf. Accessed 11 Oct 2012.
- Ruiz-Aracama, A., Lommen, A., Huber, M., Van De Vijver, L., & Hoogenboom, R. (2012). Application of an untargeted metabolomics approach for the identification of compounds that may be responsible for observed differential effects in chickens fed an organic and a conventional diet. Food Additives & Contaminants: Part A, 29, 323–332.Google Scholar
- Scott, M. L. (1966). Factors modifying the practical vitamin requirements of poultry. In Proceedings of the Cornell Nutrition Conference for Feed Manufacturers (p. 35), Ithaca, New York.Google Scholar