Metabolomics

, Volume 8, Issue 4, pp 670–678 | Cite as

Sonic vibration affects the metabolism of yeast cells growing in liquid culture: a metabolomic study

  • Raphael Bastos Mereschi Aggio
  • Victor Obolonkin
  • Silas Granato Villas-Bôas
Original Article

Abstract

It is undeniable that music and sounds can affect our emotions and mood, but so far the study of physical stimuli provoked by sound on living organisms has been mostly focused on brain and sensorimotor structures rather than cellular metabolism. Using metabolomics, we compared the physiology of yeast cells growing in defined liquid medium exposed to music, high and low frequency sonic vibration and silence. All sonic stimuli tested not only increased the growth rate of the yeast cells by 12% but they also reduced biomass production by 14%. The intra- and extracellular metabolite profiles differed significantly depending on the sonic stimulus applied showing that different metabolic pathways are affected differently by different sound frequency. Therefore, our results clearly demonstrate that sound does affect microbial cell metabolism when growing in liquid culture, opening an entirely new perspective for scientific investigation interfacing acoustics, biophysics and biochemistry.

Keywords

Metabolomics Metabolite profiling Bioacoustics Yeast physiology Cell metabolism 

References

  1. Aggio, R. B. M., Ruggiero, K., & Villas-Bôas, S. G. (2010). Pathway Activity Profiling (PAPi): From the metabolite profile to the metabolic pathway activity. Bioinformatics, 26, 2969–2976.PubMedCrossRefGoogle Scholar
  2. Aggio, R. B. M., Villas-Bôas, S. G., & Ruggiero, K. (2011). Metab: An R package for high-throughput analysis of metabolomics data generated by GC-MS. Bioinformatics, 27, 2316–2318.PubMedCrossRefGoogle Scholar
  3. Harvey, E. N., Harvey, E. B., & Loomis, A. L. (1928). Further observations on the effect of high frequency sound waves on living matter. Biological Bulletin Marine Biological Laboratory, 55, 459–469.CrossRefGoogle Scholar
  4. Harvey, E. N., & Loomis, A. L. (1928). High frequency sound waves of small intensity and their biological effects. Nature, 121, 622–624.CrossRefGoogle Scholar
  5. Jomdecha, C., & Prateepasen, A. (2006). The research of low-ultrasonic energy effects on yeast growth in fermentation process. In 12th Asia-Pacific Conference on NDT, 5th10th November 2006, Auckland, New Zealand. Google Scholar
  6. Koelsch, S., Offermanns, K., & Franzke, P. (2010). Music in the treatment of affective disorders: An exploratory investigation of a new method for music-therapeutic research. Music Perception, 27, 307–316.CrossRefGoogle Scholar
  7. Li, B., Wei, J., Tang, K., Liang, Y., Shu, K., & Wang, B. (2008). Effect of sound wave stress on antioxidant enzyme activities and lipid peroxidation of Dendrobium candidum. Colloids and Surfaces B: Biointerfaces, 63, 269–275.CrossRefGoogle Scholar
  8. Matsuhashi, M., Pankrushina, A. N., Takeuchi, S., Ohshima, H., Miyoi, H., Endoh, K., et al. (1998). Production of sound waves by bacterial cells and the response of bacterial cells to sound. The Journal of General and Applied Microbiology, 44, 49–55.PubMedCrossRefGoogle Scholar
  9. Naimark, G. M., Klair, J., & Mosher, W. A. (1951). A bibliography on sonic and ultrasonic vibration: Biological, biochemical and biophysical applications. Journal of The Franklin Institute, 251, 279–299.CrossRefGoogle Scholar
  10. Pickett, J. P., et al. (2000). The American Heritage ® Dictionary of the English Language (4th ed.). Boston: Houghton Mifflin.Google Scholar
  11. Polous, Y. U. M., & Kurko, V. S. (1991). Sound-wave stimulation of duodenal motility in chronic duodenal ileus. Klinicheskaya Meditsina, 69, 42–44.Google Scholar
  12. Sherman, F. (1997). Yeast genetics. In R. A. Meyers (Ed.), The encyclopaedia of molecular biology and molecular medicine (Vol. 6, pp. 302–325). Weinheim: VCH Publisher.Google Scholar
  13. Smart, K. F., Aggio, R. B. M., Van Houtte, J. R., & Villas-Bôas, S. G. (2010). Analytical platform for metabolome analysis microbial cells using methyl chloroformate derivatization followed by gas chromatography–mass spectrometry. Nature Protocols, 5, 1709–1729.PubMedCrossRefGoogle Scholar
  14. Syroeshkin, A. V., Bakeeva, L. E., & Cherepanov, D. A. (1998). Contraction transitions of F1–F0 ATPase during catalytic turnover. Biochimica et Biophysica Acta, 1409, 59–71.PubMedCrossRefGoogle Scholar
  15. Verduyn, C., Postma, E., Scheffers, W. A., & van Dijken, J. P. (1992). Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on regulation of respiration and alcoholic fermentation. Yeast, 8, 501–517.PubMedCrossRefGoogle Scholar
  16. Villas-Bôas, S. G., Moxley, J. F., Åkesson, M., Stephanopoulos, G., & Nielsen, J. (2005). High-throughput metabolic state analysis: The missing link in integrated functional genomics of yeasts. Biochemical Journal, 388, 669–677.PubMedCrossRefGoogle Scholar
  17. Wood, R. W., & Loomis, A. L. (1927). The physical and biological effects of high frequency sound waves of great intensity. The London, Edinburgh, and Dublin Philosophical Magazine, 4, 417–436.Google Scholar
  18. Xiujuan, W., Bochu, W., Yi, J., Defang, L., Chuanren, D., Xiaocheng, Y., et al. (2003). Effects of sound stimulation on protective enzyme activities and peroxidise isoenzymes of chrysanthemum. Colloids and Surfaces B: Biointerfaces, 27, 59–63.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Raphael Bastos Mereschi Aggio
    • 1
  • Victor Obolonkin
    • 1
  • Silas Granato Villas-Bôas
    • 1
  1. 1.Centre for Microbial Innovation, School of Biological SciencesThe University of AucklandAucklandNew Zealand

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