Lipid profiling of the model temperate grass, Brachypodium distachyon
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Lipids are essential metabolites in cells and they fulfil a variety of functions, including structural components of cellular membranes, energy storage, cell signalling, and membrane trafficking. In plants, changes in lipid composition have been observed in diverse responses ranging from abiotic and biotic stress to organogenesis. Knowledge of the lipid composition is an important first step towards understanding the function of lipids in any given biological system. As Brachypodium distachyon is emerging as the model species for temperate grass research, it is therefore fundamentally important to gain insights of its lipid composition. We used HPLC-coupled with tandem mass spectrometry to profile and quantify levels of sphingolipids and glycerophospholipids in shoots and undifferentiated cells in suspension cultures of B. distachyon. A total of 123 lipids belonging to 10 classes were identified and quantified. Our results showed that there are differences in lipid profiles and levels of individual lipid species between shoots and undifferentiated cells in suspension cultures. Additionally, we showed that 4-sphingenine (d18:1Δ4) is the main unsaturated dihydroxy-long chain base (LCB) in B. distachyon, and we were unable to detect d18:1Δ8, which is the main unsaturated dihydroxy-LCB in the model dicotyledonous species, Arabidopsis thaliana. This work serves as the first step towards a comprehensive characterization of the B. distachyon lipidome that will complement future biochemical studies.
KeywordsBrachypodium distachyon Lipidome Glycerophospholipids Sphingolipids LC–MS/MS
- Allwood, J. W., Ellis, D. I., Heald, J. K., Goodacre, R., & Mur, L. A. J. (2006). Metabolomic approaches reveal that phosphatidic acid and phosphatidyl glycerol phospholipids are major discriminatory non-polar metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea. Plant Journal, 46, 351–368.PubMedCrossRefGoogle Scholar
- Bamba, T., Shimonishi, N., Matsubara, A., Hirata, K., Nakazawa, Y., Kobayashi, A., et al. (2008). High throughput and exhaustive analysis of diverse lipids by using supercritical fluid chromatography-mass spectrometry for metabolomics. Journal of Bioscience and Bioengineering, 105, 460–469.PubMedCrossRefGoogle Scholar
- Bartke, N., Fischbeck, A., & Humpf, H.-U. (2006). Analysis of sphingolipids in potatoes (Solanum tuberosum L.) and sweet potatoes (Ipomoea batatas (L.) Lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Molecular Nutrition & Food Research, 50, 1201–1211.CrossRefGoogle Scholar
- Burgos, A., Szymanski, J., Seiwert, B., Degenkolbe, T., Hannah, M. A., Giavalisco, P., & Willmitzer, L. (2011). Analysis of short-term changes in the Arabidopsis thaliana glycerolipidome in response to temperature and light. Plant Journal. doi:10.1111/j.1365-313X.2011.04531.x.
- Garvin, D. F. (2007b). Brachypodium distachyon: A new model system for structural and functional analysis of grass genomes. In R. K. Varshney & R. M. Koebner (Eds.), Model plants & crop improvement (pp. 109–123). Boca Raton: Taylor & Francis Press.Google Scholar
- Kawaguchi, M., Imai, H., Naoe, M., Yasui, Y., & Ohnishi, M. (2000). Cerebrosides in grapevine leaves: Distinct composition of sphingoid bases among the grapevine species having different tolerances to freezing temperature. Bioscience, Biotechnology, and Biochemistry, 64, 1271–1273.PubMedCrossRefGoogle Scholar
- Markham, J. E., & Jaworski, J. G. (2007). Rapid measurement of sphingolipids from Arabidopsis thaliana by reverse-phase high performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 21, 1304–1314.PubMedCrossRefGoogle Scholar
- Michaelson, L. V., Zäuner, S., Markham, J. E. M., Haslam, R. P., Desikan, R., Mugford, S., et al. (2009). Functional characterization of a higher plant sphingolipid Δ4-desaturase: Defining the role of sphingosine and sphingosine-1-phosphate in Arabidopsis. Plant Physiology, 149, 487–498.PubMedCrossRefGoogle Scholar
- Okazaki, Y., Kamide, Y., Yokota, M., & Saito, K. (2011) Plant lipidomics based on hydrophilic interaction chromatography coupled to ion trap time-of-flight mass spectrometry. Metabolomics. doi:10.1007/s/1306-011-0318-z.