Metabolomics

, Volume 8, Issue 2, pp 323–334 | Cite as

Metabolic profiling of gender: Headspace-SPME/GC–MS and 1H NMR analysis of urine

  • Shucha Zhang
  • Lingyan Liu
  • Debora Steffen
  • Tao Ye
  • Daniel Raftery
Original Article

Abstract

This study aims to investigate the metabolic difference between male and female healthy adults using a combination of GC–MS and NMR metabolomics techniques. While metabolomics has shown wide applications in characterizing the status and progression of many diseases, physiological factors such as gender often contribute high levels of variability that can hinder the detection of biomarkers of interest, such as in disease detection. We carried out a detailed exploration of gender related metabolic profiling of human urine using a Headspace-SPME/GC–MS approach and detected over two hundred peaks. Fifty-nine metabolites were identified using the NIST library. 1H NMR spectroscopy was also utilized, and resulted in the identification of eighteen metabolites. We find that both GC–MS and NMR are able to capture human gender metabolic differences, and their combination allows a significantly better understanding of this difference. Subtle differences between genders are found to be related to the metabolism of fats, amino acids, and TCA cycle intermediates.

Keywords

Metabolomics Metabolic profiling GC/MS SPME 1H NMR Gender 

Notes

Acknowledgments

This work was supported by Purdue University. Daniel Raftery is a member of the Purdue Center for Cancer Research and Oncological Sciences Center. The authors thank all the volunteers who contributed their urine samples. Loan of GC–MS instrumentation from the Center for Authentic Science Practice in Education, Discovery Learning Center is gratefully acknowledged.

Supplementary material

11306_2011_315_MOESM1_ESM.doc (241 kb)
Supplementary material 1 (DOC 241 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Shucha Zhang
    • 2
  • Lingyan Liu
    • 1
  • Debora Steffen
    • 3
  • Tao Ye
    • 1
  • Daniel Raftery
    • 1
  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA
  2. 2.Division of Clinical ResearchFred Hutchinson Cancer Research CenterSeattleUSA
  3. 3.Discovery Learning Research Center, Purdue UniversityWest LafayetteUSA

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