, Volume 6, Issue 4, pp 497–510 | Cite as

Targeted metabolomics in an intrusive weed, Rumex obtusifolius L., grown under different environmental conditions reveals alterations of organ related metabolite pathway

  • Atsuko Miyagi
  • Kentaro Takahara
  • Hideyuki Takahashi
  • Maki Kawai-Yamada
  • Hirofumi UchimiyaEmail author
Original Article


This study was intended to analyze the metabolic pathway of Rumex obtusifolius L. (Broad-leaved dock), destructive weeds worldwide, in relation to major environmental factors (light and temperature). It was found that R. obtusifolius can be classified as plants in accumulating major organic acids such as oxalate in leaves and citrate in stems (Miyagi et al., Metabolomics 6:146–155 2010). The organ specific accumulation of certain metabolites was dissected by metabolomics approach in relation to metabolic pathway. Light or dark experiments showed that in the case of the oxalate accumulation, the major or the most dominated pathway was found to be the citrate-isocitrate-oxalate shunt. Furthermore, under the dark and/or low temperature (5°C) leaves showed sustainable growth with normal accumulation of TCA metabolites. Unlike leaves, there was a different pattern of metabolite accumulation in stems. Other metabolites such as amino acids also showed the organ specific alterations under the different ambient environments.


Rumex obtusifolius New leaves Stems Oxalate Citrate Organ specific metabolites Capillary electrophoresis mass spectrometry Principal component analysis Hierarchical clustering analysis 



This research was supported by a grant from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN) and the CREST, JST, Japan.

Supplementary material

11306_2010_220_MOESM1_ESM.pdf (24 kb)
Supplementary data Relationship between citrate and organic acids or amino acids in stems of R. obtusifolius grown under either light or dark. Data were taken from Fig. 6. W; week(s). *; P < 0.05, **; P < 0.01. (PDF 25 kb)


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Atsuko Miyagi
    • 1
    • 2
  • Kentaro Takahara
    • 2
  • Hideyuki Takahashi
    • 3
  • Maki Kawai-Yamada
    • 1
    • 4
    • 5
  • Hirofumi Uchimiya
    • 1
    • 2
    • 3
    Email author
  1. 1.Institute for Environmental Science and TechnologySaitama UniversitySaitama CityJapan
  2. 2.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan
  3. 3.Iwate Biotechnology Research CenterIwateJapan
  4. 4.Graduate School of Science and EngineeringSaitama UniversitySaitama CityJapan
  5. 5.Core Research for Evolutional Science and Technology (CREST)Japan Science and Technology Agency (JST)SaitamaJapan

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