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Metabolomics

, Volume 6, Issue 3, pp 395–404 | Cite as

The application of NMR to study changes in polar metabolite concentrations in beef longissimus dorsi stored for different periods post mortem

  • S. F. GrahamEmail author
  • T. Kennedy
  • O. Chevallier
  • A. Gordon
  • L. Farmer
  • C. Elliott
  • B. Moss
Original Article

Abstract

In this study data generated by 1H NMR spectroscopy were combined with chemometrics to analyse beef samples aged over a 21 day period. In particular, the amino acids, of which 12 were identified were found to increase over the ageing period with samples matured for 3 days having notably lower concentrations than carcasses aged for 21 days. This is believed to be a result of increased proteolysis within the muscle. This novel approach of using high resolution NMR spectrometry to analyse beef samples has not previously been reported and these findings demonstrate the potential of this technique linked with HPLC to be used as a suitable method for profiling meat samples.

Keywords

NMR Beef PCA Profiling 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • S. F. Graham
    • 1
    Email author
  • T. Kennedy
    • 2
  • O. Chevallier
    • 1
  • A. Gordon
    • 2
  • L. Farmer
    • 2
  • C. Elliott
    • 1
  • B. Moss
    • 2
  1. 1.Institute of Agri-Food and Land Use, School of Biological SciencesQueen’s University BelfastBelfastNorthern Ireland, UK
  2. 2.Agri-Food and Biosciences InstituteBelfastNorthern Ireland, UK

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