Metabolomics

, Volume 6, Issue 1, pp 109–118

Urinary metabolomics as a potentially novel diagnostic and stratification tool for knee osteoarthritis

  • Xin Li
  • Songbing Yang
  • Yunping Qiu
  • Tie Zhao
  • Tianlu Chen
  • Mingming Su
  • Lixi Chu
  • Aiping Lv
  • Ping Liu
  • Wei Jia
Original Article

DOI: 10.1007/s11306-009-0184-0

Cite this article as:
Li, X., Yang, S., Qiu, Y. et al. Metabolomics (2010) 6: 109. doi:10.1007/s11306-009-0184-0

Abstract

Metabolomics has been used as a tool in disease diagnosis and phenotype prediction. A urinary metabolomic study based on GC–MS in combination with multivariate statistics was used here to classify between knee osteoarthritis (OA) and healthy controls. OPLS-DA of the spectral data showed distinct metabolic profile variations between OA patients and healthy controls and between two OA phenotypes. Differential metabolites reveal up-regulated TCA cycle associated with OA and histamine metabolism disorders accompanied with knee effusion symptoms. This metabolomic method is potentially applicable as a novel strategy for OA diagnosis and patient stratification.

Keywords

Metabolomics Osteoarthritis Phenotype Stratification Biomarker Histamine 

Abbreviations

BMI

Body mass index

COX-2

Cyclooxygenase 2

CS

Citrate synthase

GC–MS

Gas chromatography–mass spectrometry

HAC

Human articular chondrocytes

HDC

Histidine decarboxylase

KL

Kellgren–Lawrence

MCs

Mast cells

MRI

Magnetic resonance imaging

NMR

Nuclear magnetic resonance

NSAIDs

Nonsteroidal anti-inflammatory drugs

OA

Osteoarthritis

OPLS-DA

Orthogonal partial least squares projection to latent structure-discriminant analysis

PCA

Principle component analysis

TCA

Tricarboxylic acid

TIC

Total ion current

VIP

Variable importance in the projection

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xin Li
    • 1
  • Songbing Yang
    • 2
  • Yunping Qiu
    • 1
  • Tie Zhao
    • 1
  • Tianlu Chen
    • 1
  • Mingming Su
    • 1
  • Lixi Chu
    • 3
  • Aiping Lv
    • 4
  • Ping Liu
    • 4
  • Wei Jia
    • 1
    • 5
  1. 1.School of PharmacyShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China
  2. 2.School of Acupuncture and ManipulationShanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China
  3. 3.Department of Orthopaedics and TraumatologyYueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China
  4. 4.E-Institute of Chinese Traditional Internal Medicine, Shanghai Municipal Education CommissionShanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China
  5. 5.Department of NutritionUniversity of North Carolina at GreensboroKannapolisUSA

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