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Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization

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Abstract

Suberin is a specific cell wall-associated biopolymer characterized by the deposition of both a poly(phenolic) domain (SPPD) associated with the cell wall, and a poly(aliphatic) domain (SPAD) thought to be deposited between the cell wall and plasma membrane. In planta, suberin functions to prevent plants from desiccation and pathogen attack. Although the chemical identity of the monomeric components of the SPPD and SPAD are well known, their concerted biosynthesis and assembly into the suberin macromolecule is poorly understood. To expand our knowledge of suberin biosynthesis, a GC/MS-based metabolite profiling study was conducted, using wound healing potato (Solanum tuberosum L.) tubers as a model system. A time series of both non-polar and polar metabolite profiles were created, yielding a broad-based, dynamic picture of wound-induced metabolism, including suberization. Principal component analysis revealed a separation of metabolite profiles according to different suberization stages, with clear temporal differences emerging in the non-polar and polar profiles. In the non-polar profiles, suberin-associated aliphatics contributed the most to cluster formation, while a broader range of metabolites (including organic acids, sugars, amino acids and phenylpropanoids) influenced cluster formation amongst polar profiles. Pair-wise correlation analysis revealed strong correlations between known suberin-associated compounds, as well as between suberin-associated compounds and several un-identified metabolites in the profiles. These data may help to identify additional, as yet unknown metabolites associated with suberization process.

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References

  1. Bednarek P., Franski R., Kerhoas L., Einhorn J., Wojtaszek P., Stobiecki M. (2001). Profiling changes in metabolism of isoflavonoids and their conjugates in Lupinus albus treated with biotic elicitor. Phytochemistry 56, 77–85

  2. Bernards M.A., Lewis N.G. (1992) Alkyl ferulates in wound healing potato tubers. Phytochemistry 31, 3409–3412

  3. Bernards M.A., Lewis N.G. (1998) The macromolecular aromatic domain in suberized tissue: A changing paradigm. Phytochemistry 47, 915–933

  4. Bernards M.A., Fleming W.D., Llewellyn D.B., Priefer R., Yang X., Sabatino A., Plourde G.L. (1999) Biochemical characterization of the suberin-associated anionic peroxidase of potato (Solanum tuberosum L.). Plant Physiol. 121, 135–145

  5. Bernards M.A., Susag L.M., Bedgar D.L., Anterola A.M., Lewis N.G. (2000) Induced phenylpropanoid metabolism during suberization and lignification: A comparative analysis. J. Plant Physiol. 157, 601–607

  6. Bernards M.A. (2002) Demystifying suberin. Can. J. Bot. 80, 227–240

  7. Bernards M.A., Summerhurst D.K., Razem F.A. (2003) Oxidases, peroxidases and hydrogen peroxide: The suberin connection. Phytochem. Rev. 3, 113–126

  8. Broeckling C.D., Huhman D.V., Farag M., Smith J.T., May G.D., Mendes P., Dixon R.A., Sumner L.W. (2005) Metabolic profiling of Medicago truncatula cell cultures reveals the effects of biotic and abiotic elicitors on metabolism. J. Exp. Bot. 56, 323–336

  9. Catchpole G.S., Beckmann M., Enot D.P., Mondhe M., Zywicki B., Taylor J., Hardy N., Smith A., King R.D., Kell D.B., Fiehn O., Draper J. (2005) Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops. Proc. Natl. Acad. Sci. USA 102, 14458–14462

  10. Chong J., Pierrel M.A., Atanassova R., Werck-Reithhart D., Fritig B., Saindrenan P. (2001) Free and conjugated benzoic acid in tobacco plants and cell cultures. Induced accumulation upon elicitation of defense responses and role as salicylic acid precursors. Plant Physiol. 125, 318–328

  11. Cottle W., Kolattukudy P.E. (1982) Biosynthesis, deposition, and partial characterization of potato suberin phenolics. Plant Physiol. 69, 393–399

  12. Defernez M., Gunning Y.M., Parr A.J., Shepherd L.V.T., Davies H.V., Colquhoun I.J. (2004) NMR and HPLC-UV profiling of potatoes with genetic modifications to metabolic pathways. J. Agric. Food Chem. 52, 6075–6085

  13. Dunn W.B., Bailey N.J.C., Johnson H.E. (2005) Measuring the metabolome: current analytical technologies. Analyst 130, 606–625

  14. Esau K. (1977). Anatomy of Seed Plants. John Wiley and Sons, New York

  15. Fiehn O., Kopka J., Dörmann P., Altmann T., Trethewey R.N., Willmitzer L. (2000a) Metabolite profiling for plant functional genomics. Nature Biotechnol. 18, 1157–1161

  16. Fiehn O., Kopka J., Trethewey R.N., Willmitzer L. (2000b) Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry. Anal. Chem. 72, 3573–3580

  17. Fiehn O. (2003) Metabolic networks of Cucurbita maxima phloem. Phytochemistry, 62, 875–886

  18. Graça J., Pereira H. (2000a) Methanolysis of bark suberins: analysis of glycerol and acid monomers. Phytochem. Anal. 11, 45–51

  19. Graça J., Pereira H. (2000b) Diglycerol alkendioates in suberin: building units of a poly(acylglycerol) polyester. Biomacromolecules 1, 519–522

  20. Gray G.R., Heath D. (2005) A global reorganization of the metabolome of Arabidopsis during cold acclimation is revealed by metabolic fingerprinting. Physiol. Plant. 124, 236–248

  21. Holloway P.J. (1983) Some variations in the composition of suberin from the cork layers of higher plants. Phytochemistry 22, 495–502

  22. Huhman D.V., Sumner L.W. (2002) Metabolioc profiling of saponins in Medicago sativa and Medicago truncatula using HPLC coupled to an electrospray ion-trap mass spectometer. Phytochemistry 59, 347–360

  23. Jeong M.L., Jiang H., Chen H.S., Tsai C.J., Harding S.A. (2004) Metabolic profiling of the sink-to-source transition in developing leaves of quaking aspen. Plant Physiol. 136, 3364–3375

  24. Junker B.H., Wuttke R., Nunes-Nesi A., Steinhauser D., Schauer N., Büssis D., Willmitzer L., Fernie A.R. (2006) Enhancing vacuolar sucrose cleavage within the developing potato tuber has only minor effects on metabolism. Plant Cell Physiol. 47, 277–289

  25. Kolattukudy P.E., Agrawal V.P. (1974) Structure and composition of aliphatic constituents of potato tuber skin (suberin). Lipids 9, 682–691

  26. Lopes M.H., Gil A.M., Silvestre A.D.J., Neto C.P. (2000) Composition of suberin extracted upon gradual alkaline methanolysis of Quercus suber L. cork. J. Agric. Food Chem. 48, 383–391

  27. Malmberg A. (1984) N-Feruloylputrescine in infected potato tubers. Acta Chem. Scand. B 38, 153–155

  28. Matzke K., Riederer M. (1991) A comparative study into the chemical constitution of cutins and suberins from Picea abies (L.) karst., Quercus robur L., and Fagus sylvatica L. Planta 185, 233–245

  29. Moire L., Schmutz A., Buchala A., Yan B, Stark R.E., Ryser U. (1999) Glycerol is a suberin monomer: New experimental evidence for an old hypothesis. Plant Physiol. 119, 1137–1146

  30. Razem F.A., Bernards M.A. (2002) Hydrogen peroxide is required for poly(phenolic) domain formation during wound-induced suberization. J. Agric. Food Chem. 50, 1009–1015

  31. Raamsdonk L.M., Teusink B., Broadhurst D., Zhang N., Hayes A., Walsh M.C., Berden J.A., Brindle K.M., Kell D.B., Rowland J.J, Westerho H.V., van Dam K., Oliver S.G. (2001) A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations. Nature Biotechnol. 19, 45–50

  32. Roessner U., Wagner C., Kopka J., Trethewey R.N., Willmitzer L. (2000) Simultaneous analysis of metabolites in potato tuber by gas chromatography–mass spectrometry. Plant J. 23, 131–142

  33. Roessner U., Luedemann A., Brust D., Fiehn O., Linke T., Willmitzer L., Fernie A.R. (2001a) Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell 13, 11–29

  34. Roessner U., Willmitzer L., Fernie A.R. (2001b) High-resolution metabolic phenotyping of genetically and environmentally diverse potato tuber systems. Identification of phenocopies. Plant Physiol. 127, 749–764

  35. Roessner U., Hegemann B., Lytovchenko A., Carrari F., Bruedigam C., Granot D., Fernie A.R. (2003) Metabolic profiling of transgenic tomato plants overexpressing hexokinase reveals that the influence of hexose phosphorylation diminishes during fruit development. Plant Physiol. 133, 84–99

  36. Shepherd L.V.T., McNicol J.W., Razzo R., Taylor M.A., Davies H.V. (2006) Assessing the potential for unintended effects in genetically modified potatoes perturbed in metabolic and developmental processes. Targeted analysis of key nutrients and anti-nutrients. Transgenic Res. 15, 409–425

  37. Stashenko E.E., Acosta R., Martinez J.R. (2000) High-resolution gas-chromatographic analysis of the secondary metabolites obtained by subcritical-fluid extraction from Colombian rue (Ruta graveolens L.). J. Biochem. Biophys. Method 43, 379–390

  38. Stein S.E. (1999) An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data. J. Am. Soc. Mass Spectrom. 10, 770–781

  39. Steuer R., Kurths J., Fiehn O., Weckwerth W. (2003a) Interpreting correlations in metabolomic networks. Biochem. Soc. Trans. 31, 1476–1478

  40. Steuer R., Kurth J., Fiehn O., Weckwerth W. (2003b) Observing and interpreting correlations in metabolic networks. Bioinformatics 19, 1019–1026

  41. Urbanczyk-Wochniak E., Baxter C., Kolbe A., Kopka J., Sweetlove L.J., Fernie A.R. (2005) Profiling of diurnal patterns of metabolite and transcript abundance in potato leaves reveals specific set of metabolic pathways are transcriptionally regulated, but suggests that the majority of the metabolic network is under post-transcriptional control. Planta 221, 891–903

  42. Urbanczyk-Wochniak E., Luedemann A., Kopka J., Selbig J., Roessner-Tunali U., Willmitzer L., Fernie A.R. (2003). Parallel analysis of transcript and metabolic profiles: a new approach in systems biology. EMBO Rep. 4, 989–993

  43. Weckwerth W., Loureiro M.E., Wenzel K., Fiehn O. (2004) Metabolic networks unravel the effects of silent plant phenotypes. Proc. Natl. Acad. Sci. USA 101, 7809–7814

  44. Yang W., Bernards M.A. (2006) Wound-induced metabolism in potato (Solanum tuberosum) tubers: Biosynthesis of aliphatic domain monomers. Plant Signal. Behav. 1, 59–66

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Acknowledgments

The authors gratefully acknowledge Dr. Brian McGarvey (Agriculture and Agri-Food Canada, London) for assistance with peak deconvolution and alignment and Dr. Jeff Dech (UWO) for assistance with statistical analyses. This work was funded by a Natural Sciences and Engineering Research Council of Canada Discovery Grant to MAB.

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Correspondence to Mark A. Bernards.

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Yang, W., Bernards, M.A. Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization. Metabolomics 3, 147–159 (2007). https://doi.org/10.1007/s11306-007-0053-7

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Keywords

  • suberin
  • potato
  • metabolite profiling
  • PCA
  • correlation analysis