Purinergic Signalling

, Volume 11, Issue 1, pp 79–85 | Cite as

Enhanced survival of lethally irradiated adenosine A3 receptor knockout mice. A role for hematopoietic growth factors?

  • Michal Hofer
  • Milan Pospíšil
  • Ladislav Dušek
  • Zuzana Hoferová
  • Denisa Komůrková
Original Article
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Abstract

Adenosine A3 receptor knockout (A3AR KO) mice and their wild-type (WT) counterparts were compared from the point of view of their abilities to survive exposures to lethal doses of γ-radiation belonging to the range of radiation doses inducing the bone marrow acute radiation syndrome. Parameters of cumulative 30-day survival (experiment using a midlethal radiation dose) or cumulative 11-day survival (experiment using an absolutely lethal radiation dose), and of mean survival time were evaluated. The values of A3AR KO mice always reflected their higher survival in comparison with WT ones, the P values being above the limit for statistical significance after the midlethal radiation dose and standing for statistical significance after the absolutely lethal radiation dose. This finding was considered surprising, taking into account the previously obtained findings on defects in numbers and functional properties of peripheral blood cells in A3AR KO mice. Therefore, previous hematological analyses of A3AR KO mice were supplemented in the present studies with determination of serum levels of the granulocyte colony-stimulating factor, erythropoietin, and thrombopoietin. Though distinct differences in these parameters were observed between A3AR KO and WT mice, none of them could explain the relatively high postirradiation survival of A3AR KO mice. Further studies on these mice comprising also those on other than hemopoietic tissues and organs can help to clarify their relative radioresistance.

Keywords

Adenosine A3 receptor Postirradiation survival Hematopoiesis Granulocyte colony-stimulating factor Erythropoietin Thrombopoietin 
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Notes

Acknowledgments

The authors thank the Merck Research Laboratories (West Point, PA, USA) for providing us with A3AR KO mice. This work was supported by the Grant Agency of the Czech Republic (Grant No. P303/11/0128).

Conflicts of interests

The authors declare that there are no conflicts of interests.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Michal Hofer
    • 1
  • Milan Pospíšil
    • 1
  • Ladislav Dušek
    • 2
  • Zuzana Hoferová
    • 1
  • Denisa Komůrková
    • 1
  1. 1.Department of Molecular Cytology and CytometryInstitute of Biophysics, v.v.i., Academy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Institute of Biostatistics and Analyses, Faculty of MedicineMasaryk UniversityBrnoCzech Republic

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