Comparison of fertile and sterile male gametogenesis in Cryptomeria japonica D. Don
- 58 Downloads
Cryptomeria japonica D. Don is one of the most important conifers for forestry; however, the pollinosis caused by this species is the most prevalent allergy in Japan. Male-sterile mutants have attracted attention as countermeasures to prevent pollen dispersion. Histological and transcriptomic analyses were conducted on one of the C. japonica male-sterile mutant lines, Toyama 1. Histological examination of pollen development revealed that the lamellar structure of the endexine was not observed in pollen of Toyama 1 and that the microspore was disrupted during the tetrad stage. To determine the mechanism causing male sterility in C. japonica, we analyzed gene expression in normal and sterile male strobili derived from the progeny of Toyama 1. A microarray of 22,882 low-redundancy sequences was designed from C. japonica cDNAs for this experiment. Microarray analysis revealed that the expression pattern of genes in male strobili during pollen development was very similar between fertile and male-sterile individuals. We confirmed 32 genes that were expressed at a lower level in sterile male strobili than in fertile ones at the stage when the disrupted microspores were observed in the sterile mutant. Nine of these 32 genes showed similarity to those involved in carbohydrate metabolic process, phosphorylation, and transmembrane transport. Other genes showed similarity to those involved in regulation of transcription, signal transduction, protein modification, and transport of ions and amino acids. These results suggest that the gene responsible for the Toyama 1 mutant may be involved in the network regulating pollen wall formation.
KeywordsCryptomeria japonica Male gametogenesis Male sterility Pollen development Pollen wall
This research was supported in part by a Grant-in-Aid (Development of Technologies for Control of Pollen Production by Genetic Engineering) from the Forest Agency of Japan.
- Ariizumi T, Hatakeyama K, Hinata K, Inatsugi R, Nishida I, Sato S, Kato T, Tabata S, Toriyama K (2004) Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana. Plant J 39:170–181PubMedCrossRefGoogle Scholar
- Ariizumi T, Toriyama K (2007) Pollen exine pattern formation is dependent on three major developmental processes in Arabidopsis thaliana. Int J Plant Dev Biol 1:106–115Google Scholar
- Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Statist Soc Ser B Stat Methodol 57:289–300Google Scholar
- Futamura N, Saito M, Shinohara K (2009) Transcriptional differences in male strobili between the male-sterile mutant and wild type of Cryptomeria japonica. Abstract Ann Meeting of JSPP 2009:672Google Scholar
- Moriguchi Y, Ujino-Ihara T, Uchiyama K, Futamura N, Saito M, Ueno S, Matsumoto A, Tani N, Taira H, Shinohara K, Tsumura Y (2012) The construction of a high-density linkage map for identifying SNP markers that are tightly linked to a nuclear-recessive major gene for male sterility in Cryptomeria japonica D. Don. BMC Genomics 13:95PubMedPubMedCentralCrossRefGoogle Scholar
- Nuwaysir EF, Huang W, Albert TJ, Singh J, Nuwaysir K, Pitas A, Richmond T, Gorski T, Berg JP, Ballin J, McCormick M, Norton J, Pollock T, Sumwalt T, Butcher L, Porter D, Molla M, Hall C, Blattner F, Sussman MR, Wallace RL, Cerrina F, Green RD (2002) Gene expression analysis using oligonucleotide arrays produced by maskless photolithography. Genome Res 12:1749–1755PubMedPubMedCentralCrossRefGoogle Scholar
- Oliver SN, Van Dongen JT, Alfred SC, Mamun EA, Zhao X, Saini HS, Fernandes SF, Blanchard CL, Sutton BG, Geigenberger P, Dennis ES, Dolferus R (2005) Cold-induced repression of the rice anther-specific cell wall invertase gene OSINV4 is correlated with sucrose accumulation and pollen sterility. Plant Cell Environ 28:1534–1551CrossRefGoogle Scholar
- Saito M (2008) Study on forest tree breeding for male sterility of Cryptomeria japonica D. Don. Shinrin Kagaku 54:17–20 (in Japanese)Google Scholar
- Taira H, Teranishi H, Kenda Y (1993) A case study of male sterility in sugi (Cryptomeria japonica). J Jpn For Soc 75:377–379 (in Japanese with English summary)Google Scholar
- Wilson VR, Owens JN (2003) Histology of sterile male and female cones in Pinus monticola (western white pine). Sex Plant Reprod 15:301–310Google Scholar