Single-base-resolution methylomes of Populus euphratica reveal the association between DNA methylation and salt stress
DNA methylation is an important biological form of epigenetic modification, playing key roles in plant development and environmental responses. In this study, we examined single-base resolution methylomes of Populus euphratica under control and salt stress conditions using high-throughput bisulfite sequencing. Our data showed that the methylation levels of methylated cytosines in upstream 2 kb, downstream 2 kb, and repetitive sequences increased after salt treatment in leaves, but decreased in roots. We also found that heavy methylation in 100-bp upstream of the transcriptional start site repressed gene expression, while methylations within downstream 2 K and within the gene body were positively associated with gene expression. A total of 1893 and 1817 significant differentially methylated regions (DMRs) corresponding to 251 and 191 differentially methylated genes (DMGs) were identified in leaf and root tissues, respectively. These DMGs may play important roles in salt stress responses of P. euphratica through the changes of their DNA methylation levels. Overall, these findings provide valuable insights into our understanding of the interaction between gene expression and methylation of salt responses in poplars.
KeywordsDNA methylation Populus euphratica Salt stress Gene expression
Funding for this work is provided by the National Natural Science Foundation of China (31500502), National Key Research and Development Program of China (2016YFD0600101), One Thousand Young Talents program from Sichuan Province and the Fundamental Research Funds for the Central Universities.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
Data archiving statement
The bisulfite sequencing data have been submitted to the NCBI Sequence Read Archive under accession number SRP133971. The transcriptome data is available under NCBI Sequence Read Archive with the project accession number SRP116293.
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