Genome-wide characterization of protein phosphatase 2C genes in Populus euphratica and their expression profiling under multiple abiotic stresses
- 178 Downloads
The protein phosphatase 2Cs (PP2Cs) have been demonstrated to act as negative modulators of protein kinase and to participate in stress signal transduction, as well as plant growth and productivity processes. Populus euphratica is so extraordinarily adaptable to abiotic stresses that it is regarded as a potential model plant for exploring resistance mechanisms of woody plants. To gain insight into the functional characteristics of PP2C genes in P. euphratica, 117 non-redundant PeuPP2C-encoding genes were identified from the whole genome. These members were classified into 13 groups (A–M), each of which was relatively conserved in gene structure and protein domain. A total of 39 paralogous pairs were found to be generated by whole genome duplication events, and Ka/Ks analysis indicated that these paralogous pairs had evolved mainly from purifying selection. The cis-acting elements and expression patterns showed that all the PeuPP2Cs were involved in response to single or multiple stresses including drought, salinity, heat, cold, and ABA. Taken together, our results summarized the genome-wide characterization of PeuPP2Cs and their expression profiling across different tissues and under multiple abiotic stresses in P. euphratica. These data provide a foundation to further investigate potential function of PeuPP2Cs in conferring tolerance to various stresses in P. euphratica.
KeywordsPopulus euphratica Protein phosphatase 2C (PP2C) Phylogenetic analysis Expression profiling Abiotic stress
This work was supported by National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2018ZY001-9), (CAFYBB2017ZY008), and the National Natural Science Foundation of China (31570669) to JH.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interests.
- Kim S, Park M, Yeom S, Kim YM, Lee JM, Lee HA, Seo E, Choi J, Cheong K, Kim KT, Jung K, Lee GE, Oh SK, Bae C, Kim SB, Lee HY, Kim SK, Kim MY, Kang BC, Jo TD, Yang HB, Jeong HJ, Kang WH, Kwon JK, Shin C, Lim JY, Park JH, Huh JH, Kim J, Kim B, Cohen O, Paran I, Suh MC, Lee BS, Kim YK, Shin Y, Noh SJ, Park J, Seo YS, Kwon SY, Hyun A, Kim H, Park JM, Kim HJ, Choi SB, Bosland PW, Reeves G, Jo SH, Lee BW, Cho HT, Choi HS, Lee MS, Yu Y, Choi YD, Park BS, van Deynze A, Ashrafi H, Hill T, Kim WT, Pai HS, Ahn HY, Yeam I, Giovannoni JJ, Rose JK, Sørensen I, Lee S, Ryan W Kim RW, Choi IY, Choi BS, Lim JS, Lee YH, Choi D (2014) Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nat Genet 46:270–278CrossRefPubMedGoogle Scholar
- Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu JK, Schroeder JI, Volkman BF, Cutler SR (2009) Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 324:1068–1071PubMedPubMedCentralGoogle Scholar
- Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, Schwanninger M, Kant M, Schuurink R, Mauch F, Buchala A, Cardinale F, Meskiene I (2007) The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell 19:2213–2224CrossRefPubMedPubMedCentralGoogle Scholar