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Genome-wide identification, characterization, and expression analysis of SnRK2 family in Hevea brasiliensis

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Abstract

The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) gene family belongs to a group of plant-specific serine/threonine kinase family involved in abscisic acid (ABA) signaling and biotic and abiotic stress response. Although genome-wide analyses of the SnRK2 gene family have been conducted in some species, little is known about the SnRK2 gene family in rubber tree (Hevea brasiliensis). In this study, we identified 10 SnRK2s designated as HbSnRK2.1 to HbSnRK2.10 in the rubber tree genome. The subsequently constructed phylogenetic tree demonstrated that HbSnRK2s have three subfamilies that correlate well with those of Arabidopsis sp. and rice subfamilies. All SnRK2 genes contained nine exons and eight introns. Although the C-terminus was divergent, eight conserved motifs were found. Motifs 1–6 were common to all HbSnRK2s. Expression analysis results showed that 7 of the 10 HbSnRK2s were highly expressed in latex. HbSnRK2.7 was predominantly expressed and simultaneously regulated by abscisic acid, jasmonic acid, and ethylene treatment in laticifers. HbSnRK identification and characterization provided further understanding on the role of ABA signal in the rubber tree.

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Acknowledgements

This study was supported by National Natural Science Foundation of China (No. 31471169) and Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630052016003) and the earmarked fund for China Agriculture Research System (CARS-34-ZP1).

Data archiving statement

Nucleotide sequences were deposited with the GenBank HbSnRK2.1 (GenBank accession no. KY211982), HbSnRK2.2 (GenBank accession no. KY211983), HbSnRK2.3 (GenBank accession no. KY211984), HbSnRK2.4 (GenBank accession no. KY211985), HbSnRK2.5 (GenBank accession no. KY211986), HbSnRK2.6 (GenBank accession no. KY211987), HbSnRK2.7 (GenBank accession no. KY211988), HbSnRK2.8 (GenBank accession no. KY211989), HbSnRK2.9 (GenBank accession no. KY2119990), and HbSnRK2.10 (GenBank accession no. KY211991).

Author information

Shi-Qing Peng and Dong Guo conceived and designed the experiments and drafted the manuscript; Dong Guo, Hui-Liang Li, Jia-Hong, Zhu Ying Wang, An Feng, and Gui-Shui Xie carried out the gene isolation, sequence analysis, and gene expression analysis. All authors read and approved the manuscript.

Correspondence to Shi-Qing Peng.

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The authors declare that they have no conflict of interest.

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Communicated by W. Ratnam

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Guo, D., Li, H., Zhu, J. et al. Genome-wide identification, characterization, and expression analysis of SnRK2 family in Hevea brasiliensis . Tree Genetics & Genomes 13, 86 (2017). https://doi.org/10.1007/s11295-017-1168-2

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Keywords

  • Hevea brasiliensis
  • SnRK2 family
  • Abscisic acid