High genetic similarity between Polish and North European Scots pine (Pinus sylvestris L.) populations at nuclear gene loci
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Nucleotide polymorphisms in a set of 32 nuclear genes were studied in 19 mountain, peatbog and lowland populations of Scots pine representing known phenotypic races and populations of presumably relict character for the species in Poland. At 29 genes, the pattern of genetic variation was compared to 11 reference populations from Northern, Western and Southern Europe. Similar levels of nucleotide polymorphism and excess of low-frequency mutations were observed in Polish populations (π tot = 0.0055, D = −0.308) and as compared to the reference samples (π tot = 0.0054, D = −0.170). Bayesian assignment and conventional frequency-based statistics indicate that Polish populations share the same genetic background at the analysed nuclear gene markers. However, the populations showed a much closer genetic relationship with North European samples than other regional groups of populations. Across the very uniform genetic background of the populations, we identified several genes with outlier patterns of haplotype, polymorphism frequency variation and departures from compound neutrality tests. Our data indicate that the Central and North European parts of the Scots pine distribution seem particularly suitable for association genetic studies to link phenotypic and genetic variation at a large geographical scale.
KeywordsNucleotide polymorphisms Population structure Natural selection Recolonization
The research was financially supported by the Polish Ministry of Science and Higher Education (grant nr. 2975/B/P01/2010/39). BW acknowledges the financial support from the Polish National Science Centre (grant nr. DEC-2012/05/E/NZ9/03476). We thank Euforgen network for providing the distribution map of Scots pine.
Data Archiving Statement
The nucleotide sequences of the studied loci were submitted to NCBI repositories (Acc. Nr. KJ502673-KJ503230).
- Boratyński A (1993) Systematics and geographical distribution. In: Biology of Scots pine. Edit Białobok S, Boratyński A, Bugała W (in Polish) Sorus, Poznań, KórnikGoogle Scholar
- Burczyk J, Dzialuk A, Lewandowski A (2000) Genetic variability of Scots pine (Pinus sylvestris L.) on the seed orchard in Gniewkowo (in Polish). Sylwan CXLIV (7): 65–74Google Scholar
- Derory J, Scotti-Saintagne C, Bertocchi E, Le Dantec L, Graignic N, Jauffres A, Casasoli M, Chancerel E, Bodenes C, Alberto F, Kremer A (2010) Contrasting relationships between the diversity of candidate genes and variation of bud burst in natural and segregating populations of European oaks. Heredity 104(5):438–448PubMedCrossRefGoogle Scholar
- Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1:47–50Google Scholar
- Giertych M, Oleksyn J (1992) Studies on genetic variation in Scots pine (Pinus sylvestris L.) coordinated by IUFRO. Silvae Genet 41(3):133–143Google Scholar
- Hurme P, Repo T, Savolainen O, Paakkonen T (1997) Climatic adaptation of bud set and frost hardiness in Scots pine (Pinus sylvestris). Can J For Res 27(5):716–723Google Scholar
- Naydenov K, Senneville S, Beaulieu J, Tremblay F, Bousquet J (2007) Glacial vicariance in Eurasia: mitochondrial DNA evidence from Scots pine for a complex heritage involving genetically distinct refugia at mid-northern latitudes and in Asia Minor. BMC Evol Biol 7(1):233PubMedCentralPubMedCrossRefGoogle Scholar
- Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
- Parducci L, Jørgensen T, Tollefsrud MM, Elverland E, Alm T, Fontana SL, Bennett KD, Haile J, Matetovici I, Suyama Y, Edwards ME, Andersen K, Rasmussen M, Boessenkool S, Coissac E, Brochmann C, Taberlet P, Houmark-Nielsen M, Larsen NK, Orlando L, Gilbert MTP, Kjær KH, Alsos IG, Willerslev E (2012) Glacial survival of boreal trees in northern Scandinavia. Science 335(6072):1083–1086PubMedCrossRefGoogle Scholar
- Petit RJ, Aguinagalde I, de Beaulieu J-L, Bittkau C, Brewer S, Cheddadi R, Ennos R, Fineschi S, Grivet D, Lascoux M, Mohanty A, Muller-Starck G, Demesure-Musch B, Palme A, Martin JP, Rendell S, Vendramin GG (2003) Glacial refugia: hotspots but not melting pots of genetic diversity. Science 300(5625):1563–1565PubMedCrossRefGoogle Scholar
- Sabor J (2006) Intraspecific variability of forest trees. Scots pine and Norway spruce. In: The elements of genetics and selective breeding of forest trees. Edi Sabor J (in polish) Wyd CILP, WarszawaGoogle Scholar
- Wachowiak W, Iason GR, Cavers S (2013b) Among population differentiation at nuclear genes in native Scots pine (Pinus sylvestris L.) in Scotland. Flora 208(2):79–86Google Scholar