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Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach

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Abstract

Anthocyanin-rich peaches, because of their antioxidant properties and their strong attractiveness to consumers, are increasingly considered in French peach varietal innovation programs that integrate plant genomics and classical breeding. In this study, we describe a new blood-flesh trait identified in the ‘Wu Yue Xian’ peach accession from China. ‘Wu Yue Xian’ exhibits a fully red mesocarp during the later stages of fruit development, both with green midrib leaf and normal growth of the tree. This blood-flesh phenotype clearly differs from that determined by a single recessive locus (bf) in ‘Harrow Blood’, a clone showing blood-flesh in both immature and mature fruit associated with red midrib leaf and reduced tree height. We have then provided genetic evidence that blood-flesh phenotype of ‘Wu Yue Xian’ was controlled by a single dominant locus, designated DBF (Dominant Blood-Flesh), in four successive families derived from this accession. A genetic linkage map of the blood-flesh parent (‘D6090’) of the fourth population was constructed, including 102 SSRs spanning a total distance of 562.3 cM in eight linkage groups. Whereas the bf locus is located to linkage group 4, we mapped DBF to the top of linkage group 5, thus proving that DBF and bf loci are not alleles. Among 64 predicted genes in the DBF region (505 kbp), three genes of the dihydroflavonol-4-reductase family were identified as good candidates for the control of the DBF trait. Furthermore, SSR markers flanking DBF, such as AMPP157 and AMPPG178, supply a good basis to implement marker-assisted selection for this trait.

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Acknowledgments

This work is currently funded by the European Union through the FP7 Marie Curie International Research Staff Exchange Scheme (IRSES) KBBE-246795 (STONE acronym). We are grateful to Veronique Decroocq (INRA IBVM, UMR GDPP, Villenave d'Ornon, France) who initiated the collaboration between INRA (France) and JAAS (Chine). This work also was funded by grants from the INRA through the IVD4 project established under the Convention INRA/Agri-Obtentions (InnovaFruit acronym). The authors thank the technical staff of the INRA Experimental Farm “Domaine des Pins de L’Amarine” for growing the plant material in orchard and V. Signoret for technical assistance.

Author information

Correspondence to Mingliang Yu or Thierry Pascal.

Additional information

Communicated by D. Chagné

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Shen, Z., Confolent, C., Lambert, P. et al. Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach. Tree Genetics & Genomes 9, 1435–1446 (2013). https://doi.org/10.1007/s11295-013-0649-1

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Keywords

  • Prunus persica
  • Anthocyanins
  • Mesocarp
  • SSR markers
  • Genetic map
  • Candidate gene
  • PprMYB10