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Tree Genetics & Genomes

, Volume 9, Issue 5, pp 1247–1256 | Cite as

Epigenetic characterization of chromatin in cycling cells of pedunculate oak, Quercus robur L.

  • V. Vičić
  • D. Barišić
  • T. Horvat
  • I. Biruš
  • Vlatka Zoldos
Original Paper

Abstract

Cycling cells of Quercus robur have a simple nuclear organization where most of the heterochromatin is visible as DAPI-positive chromocenters, which correspond to DAPI bands at the (peri)centromeric region of each of the 24 chromosomes of the oak complement. Immunofluorescence using 5-mC revealed dispersed distribution of the signal throughout the interphase nucleus/chromosomes without enrichment within DAPI-positive chromocenters/bands, suggesting that DNA methylation was not restricted to constitutive heterochromatin, but was associated with both euchromatic and heterochromatic domains. While H3K9ac exhibited typical euchromatin-specific distribution, the distributional pattern of histone methylation marks H3K9me1, H3K27me2, and H3K4me3 showed some specificity. The H3K9me1 and H3K27me2, both heterochromatin-associated marks, were not restricted to chromocenters, but showed additional dispersed distribution within euchromatin, while H3K27me2 mark also clustered in foci that did not co-localize with chromocenters. Surprisingly, even though H3K4me3 was distributed in the entire chromatin, many chromocenters were enriched with this euchromatin-specific modification. We discuss the distribution of the epigenetic marks in the context of the genome composition and lifestyle of Q. robur.

Keywords

Interphase chromatin Chromocenters DNA methylation Epigenetics Histone acetylation Histone methylation Quercus robur 

Notes

Acknowledgments

This work was supported by the Croatian Ministry of Science, Education and Sport Grant 119-1191196-1224 and by AUF (Agence Universitaire de Francophonie) PSCI Grant. We thank the anonymous reviewers who contributed to the improvement of this manuscript.

Supplementary material

11295_2013_632_Fig6_ESM.jpg (298 kb)
Supplementary Fig. S1

Immunolocalization of histone modification marks in A. thaliana interphase nuclei. Scale bar is 5 μm (JPEG 297 kb)

11295_2013_632_MOESM1_ESM.eps (6.8 mb)
High resolution image (EPS 6967 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • V. Vičić
    • 1
  • D. Barišić
    • 1
  • T. Horvat
    • 1
  • I. Biruš
    • 1
  • Vlatka Zoldos
    • 1
  1. 1.Department of Molecular Biology, Faculty of ScienceUniversity of ZagrebZagrebCroatia

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