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Tree Genetics & Genomes

, Volume 9, Issue 2, pp 613–619 | Cite as

Polyploidy, B chromosomes, and heterochromatin characterization of Mimosa caesalpiniifolia Benth. (Fabaceae-Mimosoideae)

  • Saulo Marçal de Sousa
  • Aryane Campos Reis
  • Lyderson Facio Viccini
Short Communication

Abstract

Eight populations of Mimosa caesalpiniifolia Benth. were investigated using a cytogenetic approach. Here, we describe for the first time details of the karyotype including chromosome morphology, physical mapping of chromomycin A3 (CMA) 4′,6-diamidino-2-phenylindole (DAPI) and silver staining of nucleolar organizer regions (Ag-NOR banding), as well as 45S rDNA sites. All populations studied showed karyotypes with 2n = 2x = 26 small metacentric and submetacentric chromosomes, although some individuals exhibited 2n = 4x = 52 chromosomes. Moreover, we observed putative additional B chromosomes in some populations. The CMA banding and fluorescent in situ hybridization techniques revealed NOR heteromorphism on the unique pair containing 45 rDNA site (chromosome 12) while the Ag-NOR banding indicated NORs on both cytotypes. Up to two and four nucleoli were observed, respectively, on individuals with 2n = 2x = 26 and 2n = 4x = 52 chromosomes and the differences in nucleolar size seems to be directly related to NOR heteromorphism in some individuals. The data present new and important information to understand karyotypic evolution of Mimosa and Fabaceae.

Keywords

Ag-NOR Chromosome banding Cytogenetics Fabaceae FISH Mimosa caesalpiniifolia 

Notes

Acknowledgments

The authors thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Conselho Nacional de Desenvolvimento Científico e Tecnológico for financial support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Saulo Marçal de Sousa
    • 1
  • Aryane Campos Reis
    • 1
  • Lyderson Facio Viccini
    • 1
  1. 1.Genetics Laboratory, Department of Biology, Institute of Biological SciencesFederal University of Juiz de ForaJuiz de ForaBrazil

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