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Tree Genetics & Genomes

, Volume 8, Issue 5, pp 1123–1134 | Cite as

Short-distance pollen dispersal for an outcrossed, wind-pollinated southern beech (Nothofagus nervosa (Phil.) Dim. et Mil.)

  • Paula Marchelli
  • Peter E. Smouse
  • Leonardo A. Gallo
Original Paper

Abstract

The mating system (outcrossing, selfing, and biparental inbreeding) and the extent of pollen flow are two of the most important genetic features that determine the genetic structure of plant populations, and both are crucial for the design of conservation strategies. The objectives here were to estimate mating system parameters and to fit the pollen dispersal kernel for the southern beech, Nothofagus nervosa. We sampled 25 mothers and 372 progeny from two stands in the Tromen Lake region of Argentina. We registered spatial positions of the maternal trees, and genotyped mothers and offspring for five simple sequence repeat markers.

We estimated single-locus (t s = 0.95) and multilocus (t m = 0.99) outcrossing rates and biparental inbreeding (t m-t s = 0.04). The species is strongly outcrossing, but correlated paternity within maternal sibships (r p = 0.10) indicates that each maternal parent is sampling a different and restricted array of pollen donors. We used two protocols (twogener and kindist) to fit an exponential power dispersal kernel to the structure of pollen clouds sampled by different mothers. The estimated effective number of pollen donors contributing to a single mother was N ep = 9.9. The twogener and kindist analyses yielded slightly different estimates, but both indicated short average distances for pollen dispersal (<35 m), indicating that the dispersal kernel was strongly leptokurtic (β = 0.36). While short-distance pollen dispersal predominates, there remains a nontrivial probability of long-distance dispersal. The results are discussed in the context of ongoing conservation and management programs.

Keywords

Gene flow Outcrossing rates kindist twogener Patagonian forests 

Notes

Acknowledgments

We thank A. Martínez Meier and M. Millerón for field assistance, A. Martínez for nursery help, M. Millerón and C. Soliani for laboratory assistance, J.E. Gayone for computational help, and J.J. Robledo-Arnuncio and F. Austerlitz for helpful discussions on data analysis. We also thank a pair of anonymous reviewers for helpful commentary of the manuscript. The study was financed by the British Ecological Society Small Project Grant No. 2258, and PIP CONICET 5451. PES was funded by NJAES/USDA-17111 and NSF-DEB-0514956.

Supplementary material

11295_2012_500_MOESM1_ESM.doc (398 kb)
ESM 1 (DOC 398 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Paula Marchelli
    • 1
    • 2
  • Peter E. Smouse
    • 3
  • Leonardo A. Gallo
    • 1
  1. 1.Grupo Genética Ecológica y Mejoramiento ForestalINTA EEA BarilocheSan Carlos de BarilocheArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y TécnicasBuenos AiresArgentina
  3. 3.Department of Ecology, Evolution and Natural Resources, School of Biological and Environmental SciencesRutgers UniversityNew BrunswickUSA

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