Tree Genetics & Genomes

, Volume 8, Issue 4, pp 775–788

Correlations of expression of cell wall biosynthesis genes with variation in biomass composition in shrub willow (Salix spp.) biomass crops

  • Michelle J. Serapiglia
  • Kimberly D. Cameron
  • Arthur J. Stipanovic
  • Lawrence B. Smart
Original Paper

Abstract

We have measured significant genetically determined variation in biomass composition among breeding populations of shrub willow, a biomass feedstock crop. This project was aimed to ask whether patterns of cell wall gene expression can be correlated with genetic variation in biomass composition at harvest, in order to develop assays of early differences in gene expression as indicators of harvestable biomass chemical composition and potentially reduce the time of selection for new willow genotypes. Previous studies have demonstrated that manipulation of expression of cell wall biosynthetic genes results in altered biomass chemical composition. We analyzed genes encoding enzymes involved in lignin biosynthesis and carbohydrate active enzymes selected based on their functional characterization and conservation in Populus trichocarpa and Arabidopsis thaliana. Fragments of 20 genes were cloned from young stem cDNA of Salix sachalinensis and Salix miyabeana. Expression profiling in willow stem apical tissue and developing stem tissue was performed for each isolated gene using probe-based quantitative real-time PCR. Two willow parental genotypes and six progeny within a hybrid family were selected for analysis, and significant differences in expression among the individuals and between tissue types were observed for most of the genes. Significant correlations between patterns of gene expression and variation in the biomass chemical composition of those genotypes provide insight into the genetic regulation of lignocellulosic deposition in this important bioenergy crop and could be utilized as a tool for early selection of new genotypes.

Keywords

Caffeic acid O-methyltransferase Caffeoyl CoA O-methyltransferase Cellulose synthase Cinnamyl alcohol dehydrogenase Glycosyltransferase Wood composition 

Supplementary material

11295_2011_462_MOESM1_ESM.pdf (389 kb)
ESM 1PDF 389 kb

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Michelle J. Serapiglia
    • 1
  • Kimberly D. Cameron
    • 1
  • Arthur J. Stipanovic
    • 2
  • Lawrence B. Smart
    • 1
  1. 1.Department of HorticultureCornell University, New York State Agricultural Experiment StationGenevaUSA
  2. 2.Department of ChemistryState University of New York College of Environmental Science and ForestrySyracuseUSA

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