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Tree Genetics & Genomes

, Volume 6, Issue 6, pp 973–980 | Cite as

Genetic and physical mapping of the SH3 region that confers resistance to leaf rust in coffee tree (Coffea arabica L.)

  • Philippe LashermesEmail author
  • Marie-Christine Combes
  • Alessandra Ribas
  • Alberto Cenci
  • Laetitia Mahé
  • Hervé Etienne
Original Paper

Abstract

Resistance to coffee leaf rust is conferred by SH3, a major dominant gene that has been introgressed from a wild coffee species Coffea liberica (genome L) into the allotetraploid cultivated species, Coffea arabica (genome CaEa). As the first step toward the map-based cloning of the SH3 gene, using a bacterial artificial chromosome (BAC) library, we describe the construction of a physical map in C. arabica spanning the resistance locus. This physical map consists in two homeologous BAC-contigs of 1,170 and 1,208 kb corresponding to the subgenomes Ca and Ea, respectively. Genetic analysis was performed using a single nucleotide polymorphism detection assay based on Sanger sequencing of amplicons. The C. liberica-derived chromosome segment that carries the SH3 resistance gene appeared to be introgressed on the sub-genome Ca. The position of the SH3 locus was delimited within an interval of 550 kb on the physical map. In addition, our results indicated a sixfold reduction in recombination frequency in the introgressed SH3 region compared to the orthologous region in Coffea canephora.

Keywords

Map-based cloning Polyploid SNP Introgression Recombination Coffee 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Philippe Lashermes
    • 1
    Email author
  • Marie-Christine Combes
    • 1
  • Alessandra Ribas
    • 1
  • Alberto Cenci
    • 1
  • Laetitia Mahé
    • 1
  • Hervé Etienne
    • 2
  1. 1.IRD - Institut de Recherche pour le DéveloppementUMR RPB (CIRAD, IRD, Université Montpellier II)Montpellier Cedex 5France
  2. 2.CIRAD–Centre de coopération internationale en recherche agronomique pour le développementUMR RPB (CIRAD, IRD, Université Montpellier II)Montpellier Cedex 5France

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