Tree Genetics & Genomes

, Volume 6, Issue 6, pp 941–952

Complete sequence and organisation of the Jatropha curcas (Euphorbiaceae) chloroplast genome

  • Mehar H. Asif
  • Shrikant S. Mantri
  • Ayush Sharma
  • Anukool Srivastava
  • Ila Trivedi
  • Priya Gupta
  • Chandra S. Mohanty
  • Samir V. Sawant
  • Rakesh Tuli
Original Paper

DOI: 10.1007/s11295-010-0303-0

Cite this article as:
Asif, M.H., Mantri, S.S., Sharma, A. et al. Tree Genetics & Genomes (2010) 6: 941. doi:10.1007/s11295-010-0303-0

Abstract

Jatropha curcas is an important non-edible oil seed tree species and is considered a promising source of biodiesel. The complete nucleotide sequence of J. curcas chloroplast genome (cpDNA) was determined by pyrosequencing and gaps filled by Sanger sequencing. The cpDNA is a circular molecule of 163,856 bp in length and codes for 110 distinct genes (78 protein coding, four rRNA and 28 distinct tRNA). Genome organisation and arrangement are similar to the reported angiosperm chloroplast genome. However, in Jatropha, the infA and the rps16 genes are non-functional. The inverted repeat (IR) boundary is within the rpl2 gene, and the 13 nucleotides at the ends of the two duplicate genes are different. Repeat analysis suggests the presence of 72 repeat regions (>30 bp) apart from the IR; of these, 48 were direct and 24 were palindromic repeats. Phylogenetic analysis of 81 protein coding chloroplast genes from 65 taxa by maximum parsimony, maximum likelihood and minimum evolution analyses at 100 bootstraps provide strong support for the placement of inaperturate crotonoids of which Jatropha is a member as sister to articulated crotonoids of which Manihot is a member.

Keywords

Jatropha curcas Chloroplast Genome Phylogeny Pyrosequencing Euphorbiaceae Angiosperms 

Supplementary material

11295_2010_303_MOESM1_ESM.doc (68 kb)
Supplementary Table 1(DOC 68 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Mehar H. Asif
    • 1
  • Shrikant S. Mantri
    • 1
  • Ayush Sharma
    • 1
  • Anukool Srivastava
    • 1
  • Ila Trivedi
    • 1
  • Priya Gupta
    • 1
  • Chandra S. Mohanty
    • 1
  • Samir V. Sawant
    • 1
  • Rakesh Tuli
    • 1
  1. 1.Plant Molecular Biology and Genetic Engineering DivisionNational Botanical Research Institute Council of Scientific and Industrial ResearchLucknowIndia

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