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Tree Genetics & Genomes

, Volume 6, Issue 2, pp 259–269 | Cite as

Clonal fingerprinting in the genus Populus L. by nuclear microsatellite loci regarding differences between sections, species and hybrids

  • Heike Liesebach
  • Volker Schneck
  • Elke Ewald
Original Paper

Abstract

Reliable methods for clone identification are desired to characterise and distinguish breeding products within the genus Populus L. (Salicaceae). Ten nuclear microsatellite loci (PMGC14, PMGC456, PMGC2163, PTR2, PTR7, WPMS05, WPMS09, WPMS14, WPMS15 and WPMS20) were applied on a clone collection with several species and hybrids belonging to the sections Tacamahaca (balsam poplars), Aigeiros (black poplars, cottonwoods) and Populus (white poplars and aspens) and intersectional hybrids between black and balsam poplars. The members of the different sections and species do not always share their allelic ladders. Some shifts of one or two nucleotides in allele length were observed for several loci. This could be explained by nucleotide sequence differences in the flanking regions of loci in diverse taxonomic groups. Such shifts of allelic ladders result in irregular patterns in hybrid genotypes. The set of loci should have a sufficient amount of variation for a differentiation between clones, even if they are full siblings originating from crossing experiments.

Keywords

Poplar Tacamahaca Aigeiros Hybrid Nuclear microsatellites Clone identification 

Notes

Acknowledgments

The research has been carried out in the vTI Institute of Forest Genetics in Waldsieversdorf, with material collected from many former colleagues. The authors thank Sarah Görß for her readily giving laboratory assistance. The authors declare that they have no conflict of interest. The careful review and attentive comments of two anonymous reviewers contributed to improve the manuscript.

Supplementary material

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Institute of Forest GeneticsJohann Heinrich von Thünen-Institut, Federal Research Institute for Rural Areas, Forestry and FisheriesWaldsieversdorfGermany

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