Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis
- 815 Downloads
Members of the MYB and MYC family regulate the biosynthesis of phenylpropanoids in several plant species. Two sequences, called CsMYB8 and CsMYC2, were identified from Citrus sinensis, and both the cDNA and the genomic clones were isolated and characterized from the flesh of common and blood oranges. Analysis by real-time polymerase chain reaction showed that the expression pattern of CsMYC2 is generally higher in rind than in flesh and in blood oranges than in common ones. In contrast, no significant difference in expression was observed for CsMYB8. The expression pattern of the structural genes chalcone synthase, anthocyanidin synthase, and UDP-glucose–flavonoid 3-O-glucosyltransferase, which code for three enzymes involved in the anthocyanin biosynthetic pathway, was also analyzed and correlated with CsMYC2, in flesh, rind, and leaf of the common and blood oranges, and in leaf of Citrus limon cultivars (characterized by anthocyanin absence or variable content). Surprisingly, CsMYC2 is highly expressed in the leaf and expression is correlated with UFGT expression in this organ. These results suggest that CsMYC2 is involved in the regulation of the flavonoid biosynthetic pathway in Citrus.
KeywordsCitrus Flavonoid Regulation
We thank Paul Bailey (John Innes Institute, Norwich) and Concetta Licciardello (C.R.A.-ACM Centro di Ricerca per l’Agrumicoltura e le Colture Mediterranee, Acireale) who constructed the phylogenetic tree. We also thank Christine Drevet (Universite Paris-XI) and Willem Van De Velde (CNRS Gif sur Yvette) for their helpful advice. This work was supported by FLORA project (Sixth Framework Programmes EEC; Contract no. 007130).
- Bagchi D, Sen CK, Bagchi M, Atalay M (2004) Anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula. Biochemistry (Mosc) 69:75–80 ReviewCrossRefGoogle Scholar
- Cotroneo PS, Russo MP, Ciuni M, Reforgiato Recupero G, Lo Piero AR (2006) Quantitative real time reverse transcriptase-PCR profiling of anthocyanin biosynthetic genes during orange fruit ripening. J Amer Soc Hort Sci 13:537–543Google Scholar
- Felsenstein J (1989) PHYLIP-Phylogeny inference package (version 3.2). Cladistics 5:164–166Google Scholar
- Jones DT, Taylor WR, Thornton JM (1992) The rapid generation of mutation data matrices from protein sequences. Comp Appl Biosci (CABIOS) 8:275–282Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning a laboratory manual, 3rd edn. Cold Spring Harbour, New YorkGoogle Scholar