Tree Genetics & Genomes

, Volume 5, Issue 1, pp 225–234 | Cite as

High-throughput genotyping and mapping of single nucleotide polymorphisms in loblolly pine (Pinus taeda L.)

  • Andrew J. Eckert
  • Barnaly Pande
  • Elhan S. Ersoz
  • Mark H. Wright
  • Vanessa K. Rashbrook
  • Charles M. Nicolet
  • David B. Neale
Original Paper

Abstract

The development and application of genomic tools to loblolly pine (Pinus taeda L.) offer promising insights into the organization and structure of conifer genomes. The application of a high-throughput genotyping assay across diverse forest tree species, however, is currently limited taxonomically. This is despite the ongoing development of genome-scale projects aiming at the construction of expressed sequence tag (EST) libraries and the resequencing of EST-derived unigenes for a diverse array of forest tree species. In this paper, we report on the application of Illumina’s high-throughput GoldenGate™ SNP genotyping assay to a loblolly pine mapping population. Single nucleotide polymorphisms (SNPs) were identified through resequencing of previously identified wood quality, drought tolerance, and disease resistance candidate genes prior to genotyping. From that effort, a 384 multiplexed SNP assay was developed for high-throughput genotyping. Approximately 67% of the 384 SNPs queried converted into high-quality genotypes for the 48 progeny samples. Of those 257 successfully genotyped SNPs, 70 were segregating within the mapping population. A total of 27 candidate genes were subsequently mapped onto the existing loblolly pine consensus map, which consists of 12 linkage groups spanning a total map distance of 1,227.6 cM. The ability of SNPs to be mapped to the same position as fragment-based markers previously developed within the same candidate genes, as well as the pivotal role that SNPs currently play in the dissection of complex phenotypic traits, illustrate the usefulness of high-throughput SNP genotyping technologies to the continued development of pine genomics.

Keywords

Loblolly pine Pinus taeda Linkage mapping Single nucleotide polymorphisms GoldenGate™ Genotyping Genomics 

Supplementary material

11295_2008_183_MOESM1_ESM.doc (124 kb)
ESM 1(DOC 127 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Andrew J. Eckert
    • 1
  • Barnaly Pande
    • 2
  • Elhan S. Ersoz
    • 3
  • Mark H. Wright
    • 4
  • Vanessa K. Rashbrook
    • 5
  • Charles M. Nicolet
    • 5
  • David B. Neale
    • 2
  1. 1.Section of Evolution and EcologyUniversity of California at DavisDavisUSA
  2. 2.Department of Plant SciencesUniversity of California at DavisDavisUSA
  3. 3.Institute for Genomic DiversityCornell UniversityIthacaUSA
  4. 4.Department of Molecular Biology and GeneticsCornell UniversityIthacaUSA
  5. 5.DNA Technologies Core Facility, Genome CenterUniversity of California at DavisDavisUSA

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