Tree Genetics & Genomes

, Volume 4, Issue 3, pp 543–554 | Cite as

Leucoanthocyanidin dioxygenase gene (PpLDOX): a potential functional marker for cold storage browning in peach

  • E. A. OgundiwinEmail author
  • C. P. Peace
  • C. M. Nicolet
  • V. K. Rashbrook
  • T. M. Gradziel
  • F. A. Bliss
  • D. Parfitt
  • C. H. Crisosto
Original Paper


Enzymatic browning of the peach fruit mesocarp is a major component of the postharvest physiological disorder commonly called chilling injury or internal breakdown (IB). Previously, we detected a major quantitative trait locus (QTL; qP-Brn5.1m) affecting browning in peach using two related progeny populations (Pop-DG and Pop-G). In this report, a gene encoding the leucoanthocanidin dioxygenase (PpLDOX) enzyme was identified as the gene potentially responsible for this QTL. PpLDOX has a high similarity with the LDOX gene of the anthocyanin biosynthesis pathway of Arabidopsis thaliana. It was co-located with qP-Brn5.1m via the bin mapping technique with the Prunus reference T×E map. A silent SNP within the PpLDOX coding sequence was used to locate the gene more precisely on the Pop-DG map and confirm its bin assignment. These results demonstrate both the utility of comparative mapping within Prunus using the T×E reference map and the power of the bin mapping approach for easily mapping genes in the Prunus genome. An SSR polymorphism was observed in the intron of PpLDOX gene sequence. The SSR co-segregated with the SNP and was used to assess association of PpLDOX with browning in 27 peach and nectarine cultivars. Cumulative evidence obtained indicates that PpLDOX partially explains genetic variation for cold storage browning susceptibility in peach and nectarine. This functional gene has potential use in marker-assisted breeding of new cultivars with lower IB susceptibility and for genotyping current cultivars for possible differential handling during storage to reduce symptom incidence.


Leucoanthocyanidin dioxygenase PpLDOX qP-Brn5.1m 



The assistance of David Garner for fruit quality measurements, Kevin Day for cultivar leaf sample collection, Josh Williamson, Jose Soto, Wai Gee and Helen Chan for laboratory assistance, and Drs. Howad and Arus for supplying the TxE bin-set DNA samples is gratefully acknowledged. This research was funded by UC Discovery Grants (bio03–10394 & bio05–10527) with the Industry-University Cooperative Research Program.


  1. Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R, Kinae N (2000) Dietry intake of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol. J Nutr 130:2378–2383Google Scholar
  2. Chagne D, Carlisle CM, Blond C, Volz RK, Whitworth CJ, Oraguzie NC, Crowhurst RN, Allan AC, Espley RV, Hellens RP, Gardiner SE (2007) Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple. BMC Genomics 8:212PubMedCrossRefGoogle Scholar
  3. Chang S, Tan C, Frankel EN, Barrett DM (2000) Low-density lipoprotein antioxidant activity of phenolic compounds and polyphenol oxidase activity in selected clingstone peach cultivars. J Agric Food Chem 48:147–151PubMedCrossRefGoogle Scholar
  4. Crisosto CH, Mitchell FG, Ju Z (1999) Susceptibility to chilling injury of peach, nectarine, and plum cultivars grown in California. HortScience 34:1116–1118Google Scholar
  5. Dirlewanger E, Graziano E, Joobeur T, Garriga-Caldere F, Cosson P, Howard W, Arus P (2004) Comparative mapping and marker assisted selection in Rosaceae fruit crops. Proc Natl Acad Sci USA 101:9891–9896PubMedCrossRefGoogle Scholar
  6. Frankel EN, Bosanek CA, Meyer AS, Silliman K, Kirk LL (1998) Commercial grape juices inhibit the in vitro oxidation of human low-density lipoprotein. J Agric Food Chem 46:834–836CrossRefGoogle Scholar
  7. Gradziel TM, Wang D (1993) Evaluation of brown rot resistance and its relation to enzymatic browning in clingstone peach germplasm. J Am Soc Hortic Sci 118:675–679Google Scholar
  8. Hansson B, Kawabe A (2005) A simple method to score single nucleotide polymorphisms based on allele-specific PCR and primer-induced fragment-length variation. Mol Ecol Notes 5:692–696CrossRefGoogle Scholar
  9. Harborne J, Williams C (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504PubMedCrossRefGoogle Scholar
  10. Hertog MGL, Hollman PCH, van de Putte B (1993) Content of potentially anticarcinogenic flavonoids in tea infusions, wine and fruit juice. Sci Food Agric 41:1242–1246CrossRefGoogle Scholar
  11. Howad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG (2005) Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map. Genetics 171:1305–1309PubMedCrossRefGoogle Scholar
  12. Iyidogan NF, Bayindirli A (2003) Effect of l-cysteine, kojic acid and 4-hexylresorcinol combination on inhibition of enzymatic browning in Amasya apple juice. J Food Eng 62:299–304CrossRefGoogle Scholar
  13. Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CWW, Fong HHS, Fransworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM (1997) Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275:218–220PubMedCrossRefGoogle Scholar
  14. Jeong SC, Saghai Maroof MA (2004) Detection and genotyping of SNPs tightly linked to two disease resistance loci, Rsv1 and Rsv3, of soybean. Plant Breeding 123:305–310CrossRefGoogle Scholar
  15. Kader AA Chordas A (1984) Evaluating the browning potential of peaches. Calif. Agr. 38:14–15Google Scholar
  16. Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res 18:999–1005PubMedCrossRefGoogle Scholar
  17. Lee M-H, Bostock RM (2006) Fruit exocarp phenols in relation to quiescence and development of Monilinia fructicola infections in Prunus: a role for cellular redox? Phytopathology 97:269–277CrossRefGoogle Scholar
  18. Lurie S, Crisosto CH (2005) Chilling injury in peach and nectarine. Postharvest Biol Technol 37:195–208CrossRefGoogle Scholar
  19. Murphy KM, Berg DG (2003) Mutation and single nucleotide polymorphism detection using temperature gradient capillary electrophoresis. Expert Rev Mol Diagno 3:811–818CrossRefGoogle Scholar
  20. Ogundiwin EA, Peace CP, Gradziel TM, Dandekar AM, Bliss FA, Crisosto CH (2007) Molecular genetic dissection of chilling injury in peach fruit. Acta Hort 738:633–638Google Scholar
  21. Peace CP, Ahmad R, Gradziel TM, Dandekar AM, Crisosto CH (2005a) The use of molecular genetics to improve peach and nectarine post-storage quality. Acta Hort 682:403–409Google Scholar
  22. Peace CP, Crisosto CH, Gradziel TM (2005b) Endopolygalacturonase: a candidate gene for Freestone and Melting flesh in peach. Mol Breeding 16:21–31CrossRefGoogle Scholar
  23. Peace CP, Crisosto CH, Garner DT, Dandekar AM, Gradziel TM Bliss FA (2006) Genetic control of internal breakdown in peach. Acta Hort 713:489–496Google Scholar
  24. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, pp 365–386Google Scholar
  25. Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witzum JL (1989) Beyond cholesterol. Modification of low-density lipoproteins that increase its atherogenicity. N Engl J Med 320:915–924PubMedCrossRefGoogle Scholar
  26. Tomas-Berberan FA, Espin JC (2001) Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 81:853–876CrossRefGoogle Scholar
  27. Tsuda T, Yamaguchi M, Honda C, Moriguchi T (2004) Expression of anthocyanin biosynthesis genes in the skin of peach and nectarine fruit. J Amer Soc Hort Sci 129:857–862Google Scholar
  28. Van Ooijen JW (2005) MapQTLÒ 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma B.V., Wageningen, NetherlandsGoogle Scholar
  29. Van Ooijen JW (2006) JoinMapÒ 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V., Wageningen, NetherlandsGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • E. A. Ogundiwin
    • 1
    • 4
    Email author
  • C. P. Peace
    • 2
  • C. M. Nicolet
    • 3
  • V. K. Rashbrook
    • 3
  • T. M. Gradziel
    • 1
  • F. A. Bliss
    • 1
  • D. Parfitt
    • 1
  • C. H. Crisosto
    • 1
  1. 1.Plant Sciences DepartmentUniversity of CaliforniaDavisUSA
  2. 2.Department of Horticulture and Landscape ArchitectureWashington State UniversityPullmanUSA
  3. 3.Genome Center, Core Services FacilitiesUniversity of CaliforniaDavisUSA
  4. 4.Plant Sciences DepartmentUC DavisKearney Agricultural CenterParlierUSA

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