Tree Genetics & Genomes

, Volume 2, Issue 4, pp 202–224

Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome

  • E. Silfverberg-Dilworth
  • C. L. Matasci
  • W. E. Van de Weg
  • M. P. W. Van Kaauwen
  • M. Walser
  • L. P. Kodde
  • V. Soglio
  • L. Gianfranceschi
  • C. E. Durel
  • F. Costa
  • T. Yamamoto
  • B. Koller
  • C. Gessler
  • A. Patocchi
Original Paper

Abstract

A new set of 148 apple microsatellite markers has been developed and mapped on the apple reference linkage map Fiesta x Discovery. One-hundred and seventeen markers were developed from genomic libraries enriched with the repeats GA, GT, AAG, AAC and ATC; 31 were developed from EST sequences. Markers derived from sequences containing dinucleotide repeats were generally more polymorphic than sequences containing trinucleotide repeats. Additional eight SSRs from published apple, pear, and Sorbus torminalis SSRs, whose position on the apple genome was unknown, have also been mapped. The transferability of SSRs across Maloideae species resulted in being efficient with 41% of the markers successfully transferred. For all 156 SSRs, the primer sequences, repeat type, map position, and quality of the amplification products are reported. Also presented are allele sizes, ranges, and number of SSRs found in a set of nine cultivars. All this information and those of the previous CH-SSR series can be searched at the apple SSR database (http://www.hidras.unimi.it) to which updates and comments can be added. A large number of apple ESTs containing SSR repeats are available and should be used for the development of new apple SSRs. The apple SSR database is also meant to become an international platform for coordinating this effort. The increased coverage of the apple genome with SSRs allowed the selection of a set of 86 reliable, highly polymorphic, and overall the apple genome well-scattered SSRs. These SSRs cover about 85% of the genome with an average distance of one marker per 15 cM.

Keywords

SSR Genetic mapping Simple sequence repeat 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • E. Silfverberg-Dilworth
    • 1
  • C. L. Matasci
    • 1
  • W. E. Van de Weg
    • 2
  • M. P. W. Van Kaauwen
    • 2
  • M. Walser
    • 1
  • L. P. Kodde
    • 2
  • V. Soglio
    • 3
  • L. Gianfranceschi
    • 3
  • C. E. Durel
    • 4
  • F. Costa
    • 5
  • T. Yamamoto
    • 6
  • B. Koller
    • 7
  • C. Gessler
    • 1
  • A. Patocchi
    • 1
    • 8
  1. 1.Plant PathologyInstitute of Integrative Biology (IBZ), ETH ZurichZurichSwitzerland
  2. 2.Department of Biodiversity and BreedingPlant Research InternationalWageningenThe Netherlands
  3. 3.Department of Biomolecular Sciences and BiotechnologyUniversity of MilanMilanItaly
  4. 4.Genetics and Horticulture (GenHort)National Institute for Agronomical Research (INRA)BeaucouzéFrance
  5. 5.Department of Fruit Tree and Woody Plant SciencesUniversity of BolognaBolognaItaly
  6. 6.National Institute of Fruit Tree ScienceTsukubaJapan
  7. 7.Ecogenics GmbHZurich-SchlierenSwitzerland
  8. 8.LFW C16ZürichSwitzerland

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