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Ecological Research

, Volume 33, Issue 4, pp 799–810 | Cite as

Crop rotation associating a legume and the nickel hyperaccumulator Alyssum murale improves the structure and biofunctioning of an ultramafic soil

  • Ramez Farajallah Saad
  • Ahmad Kobaissi
  • Gaylord Machinet
  • Geneviève Villemin
  • Guillaume Echevarria
  • Emile Benizri
Special Feature Ultramafic Ecosystems: Proceedings of the 9th International Conference on Serpentine Ecology

Abstract

Nickel (Ni) agromining aims to phytoextract heavy metals using hyperaccumulators whilst at the same time rehabilitating ultramafic soils. After removing the bioavailable metal, ultramafic soils are improved in terms of their agronomic properties with the aim of future agricultural uses. The low fertility of ultramafic soils can be compensated by integrating legumes already used in traditional agro-systems because of their importance in soil nitrogen enrichment. However, few studies have evaluated the potential profits of legumes on Ni agromining and their potential benefits on soil biological fertility. Here, we characterized the effect of a crop rotation with two plants, a legume (Vicia sativa) and a hyperaccumulator (Alyssum murale), on the phytoextraction efficiency and on soil structure and biofunctioning. A pot experiment was set up in controlled conditions to grow A. murale and four treatments were tested: rotation with V. sativa (Ro), fertilized mono-culture (FMo), non-fertilized mono-culture (NFMo) and bare soil without plants (BS). No significant difference was found between the Ro and NFMo treatments for the dry biomass yield. However, the Ro treatment showed the highest Ni concentrations ([Ni]) in A. murale shoots compared to FMo and NFMo treatments. The Ro treatment plants had more than twice as many leaves [Ni] compared to FMo. Soil physico-chemical analyses showed that the Ro treatment was better structured and showed the highest presence of bacterial micro-aggregates, as well as less non-aggregated particles. Legumes integration in Ni-agromining systems could be a pioneering strategy to reduce chemical inputs and to improve soil biofunctioning and thus fertility.

Keywords

Nickel Alyssum murale Legume Soil fertility Soil fractionation Ultrastructural characterization Agromining Crop rotation 

Notes

Acknowledgements

We would like to acknowledge the support of the technical team in the Laboratoire Sols et Environnement, Université de Lorraine. In particular, we appreciate the technical assistance of the joint Microhumus research unit and especially Maxime Maire. We would also like to thank Dr Petra Kidd and her research unit in the Consejo Superior de Investigaciones Científicas (CSIC, Santiago de Compostella, Spain) and especially the Soil Microbiology group. This work was supported by the French National Research Agency through the national ‘Investissements d’avenir’ program, ANR-10-LABX-21-LABEX RESSOURCES21, through the ANR-14-CE04-0005 project ‘Agromine’ and by the European ERA-net FACCE_SURPLUS project ‘AGRONICKEL: Developing Ni agromining on ultramafic land in Europe’, ANR-15-SUSF-0003-05. We acknowledge the Association of Specialization and Scientific Guidance (ASSG, Lebanon) for funding the PhD scholarship for Ramez Farajallah Saad and are grateful to Prof Alan Baker for his advices throughout the submission of this paper.

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© The Ecological Society of Japan 2017

Authors and Affiliations

  1. 1.Laboratoire « Sols et Environnement »Université de Lorraine, UMR 1120Vandœuvre-lès-NancyFrance
  2. 2.Laboratoire « Sols et Environnement »INRA, UMR 1120Vandœuvre-lès-NancyFrance
  3. 3.Laboratoire « Applied Plant Biotechnology », Faculté des Sciences 1Université LibanaiseBeirutLebanon
  4. 4.Laboratoire « Sols et Environnement », MicrohumusUniversité de Lorraine, UMR 1120Vandœuvre-lès-NancyFrance

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