Ecological Research

, Volume 28, Issue 4, pp 663–672 | Cite as

Geographic association and temporal variation of chemical and physical defense and leaf damage in Datura stramonium

  • Guillermo Castillo
  • Laura L. Cruz
  • Johnattan Hernández-Cumplido
  • Ken Oyama
  • César Mateo Flores-Ortiz
  • Juan Fornoni
  • Pedro L. Valverde
  • Juan Núñez-Farfán
Original Article

Abstract

The evolution of plant defense traits has traditionally been explained trough the “coevolutionary arms race” between plants and herbivores. According to this, specialist herbivores have evolved to cope effectively with the defensive traits of their host plants and may even use them as a cue for host location. We analyzed the geographic association between leaf trichomes, two tropane alkaloids (putative resistance traits), and leaf damage by herbivores in 28 populations of Datura stramonium in central Mexico. Since the specialist leaf beetles Epitrix parvula and Lema trilineata are the main herbivores of D. stramonium in central Mexico, we predicted a positive association between plant defense and leaf damage across populations. Also, if physical environmental conditions (temperature or precipitation) constrain the expression of plant defense, then the geographic variation in leaf damage should be explained partially by the interaction between defensive traits and environmental factors. Furthermore, we studied the temporal and spatial variation in leaf trichome density and leaf damage in five selected populations of D. stramonium sampled in two periods (1997 vs. 2007). We found a positive association between leaf trichomes density and atropine concentration with leaf damage across populations. The interaction between defensive traits and water availability in each locality had a significant effect on the geographic variation in leaf damage. Differences among populations in leaf trichome density are maintained over time. Our results indicate that local plant–herbivore interaction plays an important role in shaping the geographic and temporal variation in plant defense in D. stramonium.

Keywords

Datura stramonium Geographic variation Herbivory Tropane alkaloids Leaf trichomes Plant defense Solanaceae 

Notes

Acknowledgments

We thank Rosalinda Tapia López, Blanca Hernández, Martha Macías Rubalcava, María Teresa Caudillo, Luis Barbo, and Martha Urzúa Meza for laboratory assistance during HPLC quantification, and to the members of Laboratorio de Genética Ecológica y Evolución for logistic support and field assistance. We also thank the Laboratorio de Biogeoquímica FES-Iztacala and Laboratorio de Alelopatía of Instituto de Ecología, UNAM, for providing the facilities for laboratory work. This paper constitutes a partial fulfillment of the Graduate Program in Biological Sciences of the National Autonomous University of México (UNAM). GC acknowledges Lynna M. Kiere for her helpful comments on the MS and the scholarship and financial support provided by the National Council of Science and Technology (CONACyT), and UNAM. The study was funded by a CONACyT grant 81490 “Evolución de la defensa en plantas contra sus enemigos naturales” to JNF.

Supplementary material

11284_2013_1059_MOESM1_ESM.doc (60 kb)
Supplementary material 1 (DOC 59 kb)
11284_2013_1059_MOESM2_ESM.tif (452 kb)
S 2. Interaction surfaces between predictor variables resulting from the multiple regression model. a Leaf atropine concentration (in 2.5 mm2) × Lang’s Index (mg/g), and b Leaf scopolamine concentration × mean population plant size. Response variable was the proportion leaf damage. (TIFF 452 kb)
11284_2013_1059_MOESM3_ESM.doc (34 kb)
Supplementary material 3 (DOC 34 kb)

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Copyright information

© The Ecological Society of Japan 2013

Authors and Affiliations

  • Guillermo Castillo
    • 1
  • Laura L. Cruz
    • 1
  • Johnattan Hernández-Cumplido
    • 2
  • Ken Oyama
    • 3
  • César Mateo Flores-Ortiz
    • 4
  • Juan Fornoni
    • 1
  • Pedro L. Valverde
    • 5
  • Juan Núñez-Farfán
    • 1
  1. 1.Laboratorio de Genética Ecológica y Evolución, Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexicoMexico
  2. 2.Laboratory of Evolutionary Entomology, Institute of BiologyUniversity of Neuchâtel (UNINE)NeuchâtelSwitzerland
  3. 3.Centro de Investigaciones en EcosistemasUniversidad Nacional Autónoma de México (UNAM)MoreliaMexico
  4. 4.Laboratorio de Fisiología Vegetal, UBIPROUniversidad Nacional Autónoma de México Facultad de Estudios Superiores IztacalaMexicoMexico
  5. 5.Departamento de BiologíaUniversidad Autónoma Metropolitana-IztapalapaMexicoMexico

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