Ecological Research

, Volume 20, Issue 2, pp 233–238

Genetic structure of Miscanthus sinensis ssp. condensatus (Poaceae) on Miyake Island: implications for revegetation of volcanically devastated sites

  • Hiroyoshi Iwata
  • Takashi Kamijo
  • Yoshihiko Tsumura
Note and Comment

Abstract

We investigated the genetic structure of Miscanthus sinensis ssp. condensatus on Miyake Island, which was devastated by a volcanic eruption in 2000, by amplified fragment length polymorphisms (AFLPs) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for chloroplast DNA (cpDNA) variation, to develop recommendations for the revegetation of devastated sites. Genetic differentiation among populations was significant, and five populations were classified into three regional groups. The aspect ratios of leaf-blades varied significantly among populations, but both geographical proximity and morphological similarities did not precisely reflect genetic similarities. In the airport population, we found a rare haplotype that may have been transmitted from outside the island. These findings will assist the revegetation of the island.

Keywords

Revegetation Genetic differentiation Chloroplast DNA (cpDNA) Amplified fragment length polymorphisms (AFLPs) PCR-RFLP 

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Copyright information

© The Ecological Society of Japan 2004

Authors and Affiliations

  • Hiroyoshi Iwata
    • 1
  • Takashi Kamijo
    • 2
  • Yoshihiko Tsumura
    • 3
  1. 1.Department of Information Science and TechnologyNational Agricultural Research CenterTsukuba, IbarakiJapan
  2. 2.Institute of Agriculture and ForestryUniversity of TsukubaTsukuba, IbarakiJapan
  3. 3.Department of Forest GeneticsForestry and Forest Products Research InstituteTsukuba, IbarakiJapan

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