Effects of citric acid administration on femoral trabecular structures in ovariectomized mice
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To examine the effects of citric acid administration on trabecular structures.
Ovariectomized female ICR mice (n = 39), 8 weeks of age, were divided into four groups: ovariectomized control (OVX-control, n = 9, standard diet); citric acid (CIT, n = 10, citric acid 5 g/100 g diet); vitamin K2 (menaquinone 7; VK2, n = 10, vitamin K2 50 µg/100 g diet); and citric acid + vitamin K2 (CIT + VK2, n = 10, citric acid 5 g/100 g diet and vitamin K2 50 µg/100 g diet). After 12 weeks, the bone mass of the right femur was measured using peripheral quantitative computed tomography and the three-dimensional (3-D) trabecular structure of the right femur was assessed using microfocus computed tomography.
The bone mineral density was significantly increased in the VK2 group versus the OVX-control group (P < 0.05). In the 3-D trabecular structure analysis, skeletal perimeter and number were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Skeletal separation, spacing, and trabecular bone pattern factor were significantly lower in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Almost all parameters in node–strut analysis were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group.
These results suggest that citric acid improved trabecular structures. However, simultaneous administration of citric acid and vitamin K2 did not have any additional effect.
Key wordsOsteoporosis Mice Citric acid Vitamin K2
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