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Valinomycin, produced by Streptomyces sp. S8, a key antifungal metabolite in large patch disease suppressiveness

  • Chang-Wook Jeon
  • Da-Ran Kim
  • Youn-Sig KwakEmail author
Original Paper

Abstract

Large patch disease, caused by Rhizoctonia solani AG2-2, is the most devastating disease in Zoysiagrass (Zoysia japonica). Current large patch disease control strategies rely primarily upon the use of chemical pesticides. Streptomyces sp. S8 is known to possess exceptional antagonistic properties that could potentially suppress the large patch pathogen found at turfgrass plantations. This study aims to demonstrate the feasibility of using the strain as a biological control mechanism. Sequencing of the S8 strain genome revealed a valinomycin biosynthesis gene cluster. This cluster is composed of the vlm1 and vlm2 genes, which are known to produce antifungal compounds. In order to verify this finding for the large patch pathogen, a valinomycin biosynthesis knockout mutant was created via the CRISPR/Cas9 system. The mutant lost antifungal activity against the large patch pathogen. Consequently, it is anticipated that eco-friendly microbial preparations derived from the S8 strain can be utilized to biologically control large patch disease.

Keywords

Biological control Large patch Streptomyces Valinomycin Zoysiagrass 

Notes

Acknowledgement

This work was supported by the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Project No. 315004-5).

Supplementary material

11274_2019_2704_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 24 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Dvision of Applied Life Science (BK21Plus)Gyeongsang National UniversityJinjuSouth Korea
  2. 2.Department of Plant Medicine and Institute of Agriculture & Life SciencesGyeongsang National UniversityJinjuSouth Korea

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