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Polar flagellum of the alphaproteobacterium Azospirillum brasilense Sp245 plays a role in biofilm biomass accumulation and in biofilm maintenance under stationary and dynamic conditions

  • Andrei V. Shelud’ko
  • Yulia A. Filip’echeva
  • Elizaveta M. Telesheva
  • Stella S. Yevstigneeva
  • Lilia P. Petrova
  • Elena I. KatsyEmail author
Original Paper
  • 51 Downloads

Abstract

Bacteria Azospirillum brasilense may swim and swarm owing to the rotation of a constitutive polar flagellum (Fla) and inducible lateral flagella (Laf). They also construct sessile biofilms on various interfaces. As compared to the wild-type strain Sp245, the previously characterized Fla Laf flhB1 mutant Sp245.1063 accumulated less biomass in mature biofilms, which also were susceptible to the forces of hydrodynamic shear. In this study, we compared biofilms formed by strain Sp245 and its previously constructed derivatives on the interfaces between a minimal (malate–salt medium, or MSM) or rich (LB) liquid growth medium and a hydrophilic (glass) or hydrophobic (polystyrene) solid surface under static or dynamic conditions. In all experimental settings, the alterations in Sp245.1063’s mature biofilm traits were partially (in MSM) or completely (in LB) rescued in the complemented mutant Sp245.1063 (pRK415–150177), which received the pRK415-borne coding sequence for the putative FlhB1 protein of the flagellar type III secretion system. Although Laf were not found in the biofilms of azospirilla, Fla was present on the biofilm cells of the complemented mutant Sp245.1063 (pRK415–150177) and other studied strains, which had normal flagellation on liquid and solid nutritional media. Accordingly, mature biofilms of these strains contained more biomass and were significantly more resistant to shaking at 140 rpm, as compared to the biofilms of the flagella-free mutant bacteria. These data proved that the polar flagellum of A. brasilense Sp245 plays a significant positive role in biofilm biomass increase and in biofilm stabilization.

Keywords

Azospirillum brasilense Polar flagellum Biofilm Hydrodynamic shear 

Notes

Acknowledgements

The authors are grateful to Dr. A.M. Burov for expert technical support and to the Symbiosis Center for the Collective Use of Research Equipment in the Field of Physical–Chemical Biology and Nanobiotechnology at the IBPPM RAS (Saratov, Russia) for providing access to research equipment.

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest regarding the publication of this article.

Supplementary material

11274_2019_2594_MOESM1_ESM.pdf (1.2 mb)
Supplementary material 1 (PDF 1184 KB)

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia

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