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Immobilization pattern of morphologically different microorganisms on bacterial cellulose membranes

  • Anna Żywicka
  • Karolina Wenelska
  • Adam Junka
  • Grzegorz Chodaczek
  • Patrycja Szymczyk
  • Karol Fijałkowski
Original Paper
  • 64 Downloads

Abstract

The aim of this study was to assess the immobilization pattern of microorganisms characterized by varying cell shapes and sizes (rod-shaped bacteria Lactobacillus delbruecki, spherical-shaped yeast Saccharomyces cerevisiae and hyphae forms of Yarrowia lipolytica) on bacterial cellulose of various material properties. The ‘adsorption-incubation’ method was used for the purposes of immobilization. The immobilization pattern included adsorption efficiency, ability of the immobilized cells to multiply within the carrier expressed as incubation efficiency and the degree of release of the immobilized cells from the carrier. The efficiency of adsorption and incubation was affected by the morphology of the immobilized cells and increased together with cellulose surface area. For smaller bacterial cells a higher level of loading was obtained on the same surface as compared to larger yeast cells. During incubation, the number of immobilized bacterial and yeast cells increased significantly in comparison to the number of cells adsorbed on the carrier during the adsorption step. Despite the morphological differences between the S. cerevisiae and Y. lipolytica cells, there were no statistically significant differences in the efficiency of adsorption and incubation. It was also revealed that the release ratio values obtained for L. delbruecki and S. cerevisiae increased along with cellulose surface area. Interestingly, Y. lipolytica cells in the pseudohyphae and hyphae forms penetrated deeply into the three-dimensional network of BC nanofibrils which prevented subsequent cell release. It was confirmed that carrier selection must be individually matched to the type of immobilized cells based especially on its porosity-related parameters.

Graphical abstract

Keywords

Bacterial cellulose Efficiency of adsorption, efficiency of incubation Microorganism Release ratio 

Notes

Acknowledgements

The authors would like to thank the Dean of the Faculty of Biotechnology and Animal Husbandry (Grant No. 517-01-027-3323/17) and the National Centre for Research and Development in Poland (Grant No. LIDER/011/221/L-5/13/NCBR/2014) for providing financial support to this work.

Supplementary material

11274_2018_2584_MOESM1_ESM.docx (164 kb)
Supplementary material 1 (DOCX 163 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal HusbandryWest Pomeranian University of Technology, SzczecinSzczecinPoland
  2. 2.Nanomaterials Physicochemistry Department, Faculty of Chemical Technology and EngineeringWest Pomeranian University of Technology, SzczecinSzczecinPoland
  3. 3.Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy with Division of Laboratory DiagnosticsWroclaw Medical UniversityWrocławPoland
  4. 4.Laboratory of Confocal MicroscopyWrocław Research Centre EIT+WrocławPoland
  5. 5.Center for Advanced Manufacturing Technologies (CAMT/FPC), Faculty of Mechanical EngineeringWroclaw University of Science and TechnologyWrocławPoland

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