Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis

  • Kun-Qiang Hong
  • Ding-Yu Liu
  • Tao Chen
  • Zhi-Wen WangEmail author


Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR) system, which uses an easily customizable specificity RNA-guided DNA endonuclease, such as Cas9. Recently, CRISPR/Cas9 mediated genome engineering has been widely applied to model organisms, including Bacillus subtilis, enabling facile, rapid high-fidelity modification of endogenous native genes. Here, we reviewed the recent progress in B. subtilis gene editing using CRISPR/Cas9 based tools, and highlighted state-of-the-art strategies for design of CRISPR/Cas9 system. Finally, future perspectives on the use of CRISPR/Cas9 genome engineering for sequence-specific genome editing in B. subtilis are provided.


Bacillus subtilis CRISPR/Cas9 Genome editing High-throughout 



This work was supported by National Natural Science Foundation of China (Grant Nos. NSFC-21576200, NSFC-21776209, and NSFC-21621004).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

For this type of study formal consent is not required.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Kun-Qiang Hong
    • 1
    • 2
    • 3
  • Ding-Yu Liu
    • 1
    • 2
    • 3
  • Tao Chen
    • 1
    • 2
    • 3
  • Zhi-Wen Wang
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Biochemical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinPeople’s Republic of China
  2. 2.Key Laboratory of Systems Bioengineering (Ministry of Education)Tianjin UniversityTianjinPeople’s Republic of China
  3. 3.SynBio Research PlatformCollaborative Innovation Center of Chemical Science and Engineering (Tianjin)TianjinPeople’s Republic of China

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