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Efficient production of human zona pellucida-3 in a prokaryotic expression system

  • Zhuolin Qiu
  • Mingzhu Wang
  • Miaoqin She
  • Qianmei Chen
  • Qingqing Li
  • Jianbin Zhu
  • Xiaorong YangEmail author
  • Xiangsheng CaiEmail author
Original Paper
  • 156 Downloads

Abstract

The zona pellucida-3 (ZP3) protein plays a pivotal role in oocyte and gamete development. We aimed to produce a recombinant ZP3 peptide using the Escherichia coli secretory system and apply it to a protein chip for detecting anti-ZP3 antibodies. The ZP3 gene was cloned into the pHOA downstream of the phoA promoter and transformed into E. coli YK537. Recombinant ZP3 was secretory expressed by decreasing the inorganic phosphate concentration. Then, rZP3 was purified and coated onto a protein chip, which was used to detect AZP3A in serum samples from 63 infertile patients. The area under the receiver operating characteristic curve was 0.934. The results, in terms of AZP3A detection, of the rZP3-coated protein chip were consistent with those of the ELISA kit. Therefore, our protein chip assay has potential for diagnosis of infertility due to AZP3A, and represents a less costly and simpler assay for clinical and research applications.

Keywords

Zona pellucida-3 PhoA Protein chip Secretory expression Infertility AZP3A 

Notes

Acknowledgements

We gratefully thank Guangdong Province Science and Technology Fund (No: 2017ZC0208) for their financial support.

Compliance with ethical standards

Conflict of interest

The authors have declared that no conflict of interest exist.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Zhuolin Qiu
    • 1
  • Mingzhu Wang
    • 2
  • Miaoqin She
    • 1
  • Qianmei Chen
    • 1
  • Qingqing Li
    • 1
  • Jianbin Zhu
    • 3
  • Xiaorong Yang
    • 4
    Email author
  • Xiangsheng Cai
    • 1
    • 4
    Email author
  1. 1.Reproductive Center of Obstetrics and GynecologySouthern Medical UniversityGuangzhouPeople’s Republic of China
  2. 2.Center of Reproduction Medicine in Fourth Hospital of Xi’an CityXi’anPeople’s Republic of China
  3. 3.Technology CenterGuangdong Vitalife Bio-Tech Co., LTDFoshanPeople’s Republic of China
  4. 4.Clinical Laboratory and Medical Laboratory DepartmentThe First Affiliated Hospital of Guangdong Pharmaceutical UniversityGuangzhouPeople’s Republic of China

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