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Antibacterial evaluation of silver nanoparticles synthesized from lychee peel: individual versus antibiotic conjugated effects

  • Shaghufta Perveen
  • Naila Safdar
  • Gul-e-saba Chaudhry
  • Azra Yasmin
Original Paper

Abstract

This paper describes the extracellular synthesis of silver nanoparticles from waste part of lychee fruit (peel) and their conjugation with selected antibiotics (amoxicillin, cefixim, and streptomycin). FTIR studies revealed the reduction of metallic silver and stabilization of silver nanoparticles and their conjugates due to the presence of CO (carboxyl), OH (hydroxyl) and CH (alkanes) groups. The size of conjugated nanoparticles varied ranging from 3 to 10 nm as shown by XRD. TEM image revealed the spherical shape of biosynthesized silver nanoparticles. Conjugates of amoxicillin and cefixim showed highest antibacterial activity (147.43 and 107.95%, respectively) against Gram-negative bacteria i.e. Alcaligenes faecalis in comparison with their control counterparts. The highest reduction in MIC was noted against Gram-positive strains i.e. Enterococcus faecium (75%) and Microbacterium oxydans (75%) for amoxicillin conjugates. Anova two factor followed by two-tailed t test showed non-significant results both in case of cell leakage and protein estimation between nanoparticles and conjugates of amoxicillin, cefixime and streptomycin. In case of MDA release, non-significant difference among the test samples against the selected strains. Our study found green-synthesized silver nanoparticles as effective antibacterial bullet against both Gram positive and Gram negative bacteria, but they showed a more promising effect on conjugation with selected antibiotics against Gram negative type.

Keywords

Silver nanoparticles Lychee peel Antibacterial evaluation Antibiotics conjugates Protein estimation Malondialdehyde contents 

Supplementary material

11274_2018_2500_MOESM1_ESM.docx (457 kb)
Supplementary material 1 (DOCX 457 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Shaghufta Perveen
    • 1
  • Naila Safdar
    • 1
  • Gul-e-saba Chaudhry
    • 2
  • Azra Yasmin
    • 1
  1. 1.Microbiology and Biotechnology Research LabFatima Jinnah Women UniversityRawalpindiPakistan
  2. 2.Institute of Marine BiotechnologyUniversiti Malaysia, TerengganuKuala TerengganuMalaysia

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