Antibacterial evaluation of silver nanoparticles synthesized from lychee peel: individual versus antibiotic conjugated effects

  • Shaghufta Perveen
  • Naila SafdarEmail author
  • Gul-e-saba Chaudhry
  • Azra Yasmin
Original Paper


This paper describes the extracellular synthesis of silver nanoparticles from waste part of lychee fruit (peel) and their conjugation with selected antibiotics (amoxicillin, cefixim, and streptomycin). FTIR studies revealed the reduction of metallic silver and stabilization of silver nanoparticles and their conjugates due to the presence of CO (carboxyl), OH (hydroxyl) and CH (alkanes) groups. The size of conjugated nanoparticles varied ranging from 3 to 10 nm as shown by XRD. TEM image revealed the spherical shape of biosynthesized silver nanoparticles. Conjugates of amoxicillin and cefixim showed highest antibacterial activity (147.43 and 107.95%, respectively) against Gram-negative bacteria i.e. Alcaligenes faecalis in comparison with their control counterparts. The highest reduction in MIC was noted against Gram-positive strains i.e. Enterococcus faecium (75%) and Microbacterium oxydans (75%) for amoxicillin conjugates. Anova two factor followed by two-tailed t test showed non-significant results both in case of cell leakage and protein estimation between nanoparticles and conjugates of amoxicillin, cefixime and streptomycin. In case of MDA release, non-significant difference among the test samples against the selected strains. Our study found green-synthesized silver nanoparticles as effective antibacterial bullet against both Gram positive and Gram negative bacteria, but they showed a more promising effect on conjugation with selected antibiotics against Gram negative type.


Silver nanoparticles Lychee peel Antibacterial evaluation Antibiotics conjugates Protein estimation Malondialdehyde contents 

Supplementary material

11274_2018_2500_MOESM1_ESM.docx (457 kb)
Supplementary material 1 (DOCX 457 KB)


  1. Ahmad N, Sharma S (2012) Green synthesis of silver nanoparticles using extract of ananas comosus. Green Sustain Chem 2(4):1–7. CrossRefGoogle Scholar
  2. Batarseh KI (2004) Anomaly and correlation of killing in the therapeutic properties of silver (I) chelation with glutamic and tartaric acids. J Antimicrob Chemother 54(2):546–548. CrossRefPubMedGoogle Scholar
  3. Da Silva LCN, de Cassia Mendonccedil R, de Barros Gomes E, de Araujo JM, de Figueiredo RCBQ, da Silva MV, dos Santos Correia MT (2013) Evaluation of combinatory effects of Anadenanthera colubrina, Libidibia ferrea and Pityrocarpa moniliformis fruits extracts and erythromycin against Staphylococcus aureus.. J Med Plant Res 7(32):2358–2364. CrossRefGoogle Scholar
  4. Dutta RK, Nenavathu BP, Gangishetty MK, Reddy AVR (2012) Studies on antibacterial activity of ZnO nanoparticles by ROS induced lipid peroxidation. Colloids Surf B 94:143–150. CrossRefGoogle Scholar
  5. Govindaraju K, Tamilselvan S, Kiruthiga V, Singaravelu G (2010) Biogenic silver nanoparticles by Solanum torvum and their promising antimicrobial activity. J Biopesticides 3(1):394–399Google Scholar
  6. Gurunathan S (2014) Rapid biological synthesis of silver nanoparticles and their enhanced antibacterial effects against Escherichia fergusonii and Streptococcus mutans. Arab J Chem. CrossRefGoogle Scholar
  7. Harshiny M, Matheswaran M, Arthananreeswaran G, Kumaran S, Rajasree S (2015) Enhancement of antibacterial properties of silver nanoparticles–ceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis. Ecotoxicol Environ Saf 121:135–141. CrossRefPubMedGoogle Scholar
  8. Konop M, Damps T, Misicka A, Rudnicka L (2016) Certain aspects of silver and silver nanoparticles in wound care: a minireview. J Nanomater. CrossRefGoogle Scholar
  9. Krishna G, Kumar SS, Pranitha V, Alha M, Charaya S (2015) Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against Salmonella sp. Int J Pharm Pharm Sci 7(11):84–88Google Scholar
  10. Miller KP (2015) Bacterial communication and its role as a target for nanoparticle-based antimicrobial therapy. Doctoral dissertation.
  11. Mohammadi S, Pourseyedi S, Amini A (2016) Green synthesis of silver nanoparticles with a long lasting stability using colloidal solution of cowpea seeds (Vigna sp. L). J Environ Chem Eng 4(2):2023–2032. CrossRefGoogle Scholar
  12. Mu H, Tang J, Liu Q, Sun C, Wang T, Duan J (2016) Potent antibacterial nanoparticles against biofilm and intracellular bacteria. Sci Rep 6:18877. CrossRefPubMedPubMedCentralGoogle Scholar
  13. Nisha HM, Tamileaswari R, Jesurani S (2015) Analysis of antibacterial activity of silver nanoparticle from pomegranate (Punica granatum) seed and peel extract. Int J Eng Res Technol 4(4):1044Google Scholar
  14. Nowack B, Krug HF, Height M (2011) 120 years of nanosilver history: implications for policy makers. Environ Sci Technol 44:1177–1183. CrossRefGoogle Scholar
  15. Pal S, Tak KY, Song MJ (2007) Does the antibacterial activity of silver 248 nanoparticles depend on the shape of the nanoparticle? A study of the 249 Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73(6):1712–1720CrossRefPubMedPubMedCentralGoogle Scholar
  16. Pubchem (2016a) Amoxicillin from National Center for Biotechnology Information. PubChem Compound Database. Accessed 6 Mar 2016
  17. Pubchem (2016b) CEFIXIME from National Center for Biotechnology Information. PubChem Compound Database. Accessed 6 Mar 2016
  18. Queiroz EDR, Abreu CMPD, Oliveira KDS, Ramos VDO, Fráguas RM (2015) Bioactive phytochemicals and antioxidant activity in fresh and dried lychee fractions1. Revista Ciência Agronômica 46(1):163–169. CrossRefGoogle Scholar
  19. Ramteke C, Chakrabarti T, Sarangi KB, Pandey AR (2013) Synthesis of silver nanoparticles from the aqueous extract of leaves of Ocimum sanctum for enhanced antibacterial activity. J Chem. CrossRefGoogle Scholar
  20. Rao ML, Bhumi G, Savithramma N (2013) Green synthesis of silver nanoparticles by Allamanda cathartica L. leaf extract and evaluation for antimicrobial activity. Int J Pharm Sci Nanotechnol 6(4):2260–2268Google Scholar
  21. Raza MA, Kanwal Z, Rauf A, Sabri AN, Riaz S, Naseem S (2016) Size-and shape-dependent antibacterial studies of silver nanoparticles synthesized by wet chemical routes. Nanomaterials 6(4):74CrossRefPubMedCentralGoogle Scholar
  22. Reddy DHK, Seshaiah K, Reddy AVR, Lee SM (2012) Optimization of Cd (II), Cu (II) and Ni (II) biosorption by chemically modified Moringa oleifera leaves powder. Carbohydr Polym 88(3):1077–1086CrossRefGoogle Scholar
  23. Reenal M, Iruthaya KSS (2015) Green synthesis and antibacterial activity of silver nanoparticles using Oryza sativa husk extract. Int Res J Environ Sci 4(5):68–72Google Scholar
  24. Rigo C, Ferroni L, Tocco I, Roman M, Munivrana I, Gardin C et al (2013) Active silver nanoparticles for wound healing. Int J Mol Sci 14(3):4817–4840. CrossRefPubMedPubMedCentralGoogle Scholar
  25. Shukla SK, Chaudhary P, Kumar IP, Samanta N, Afrin F, Gupta ML et al (2006) Protection from radiation-induced mitochondrial and genomic DNA damage by an extract of Hippophae rhamnoides. Environ Mol Mutagen 47(9):647–656. CrossRefPubMedGoogle Scholar
  26. Singh P, Kumar R, Raja BR, Kalaichelvan TP (2011) Mycobased biosynthesis of silver nanoparticles and studies of its synergistic antibacterial activity combined with cefazolin antibiotic against selected organisms. Aust J Basic Appl Sci 5(8):1412–1427Google Scholar
  27. Ul Ain N, Safdar N, Yasmin A (2017) Antimicrobial Investigations from crude and peptide extracts of Glycine max Linn. Merr varieties. Arab J Sci Eng 42(1):105–113. CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Shaghufta Perveen
    • 1
  • Naila Safdar
    • 1
    Email author
  • Gul-e-saba Chaudhry
    • 2
  • Azra Yasmin
    • 1
  1. 1.Microbiology and Biotechnology Research LabFatima Jinnah Women UniversityRawalpindiPakistan
  2. 2.Institute of Marine BiotechnologyUniversiti Malaysia, TerengganuKuala TerengganuMalaysia

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