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Rhodococcus bacteria as a promising source of oils from olive mill wastes

  • O. Marisa Herrero
  • María S. Villalba
  • Mariana P. Lanfranconi
  • Héctor M. Alvarez
Original Paper
  • 147 Downloads

Abstract

The accumulation of triacylglycerols (TAG) is a common feature among actinobacteria belonging to Rhodococcus genus. Some rhodococcal species are able to produce significant amounts of those lipids from different single substrates, such as glucose, gluconate or hexadecane. In this study we analyzed the ability of different species to produce lipids from olive oil mill wastes (OMW), and the possibility to enhance lipid production by genetic engineering. OMW base medium prepared from alperujo, which exhibited high values of chemical oxygen demand (127,000 mg/l) and C/N ratio (508), supported good growth and TAG production by some rhodococci. R. opacus, R. wratislaviensis and R. jostii were more efficient at producing cell biomass (2.2–2.7 g/l) and lipids (77–83% of CDW, 1.8–2.2 g/l) from OMW than R. fascians, R. erythropolis and R. equi (1.1–1.6 g/l of cell biomass and 7.1–14.0% of CDW, 0.1–0.2 g/l of lipids). Overexpression of a gene coding for a fatty acid importer in R. jostii RHA1 promoted an increase of 2.2 fold of cellular biomass value with a concomitant increase in lipids production during cultivation of cells in OMW. This study demonstrates that the bioconversion of OMW to microbial lipids is feasible using more robust rhodococal strains. The efficiency of this bioconversion can be significantly enhanced by engineering strategies.

Keywords

Olive mill wastes Bioconversion Lipid production Engineered Rhodococcus 

Notes

Acknowledgements

The technical assistance of Enrique Rost for GC analyses and Pedro Torrecillas for chemical oxygen demand analyses is gratefully acknowledged.

Funding

This work was funded by Projects PIP2015-16 Nro 0529, PICT2012 Nro 2031, and Oil m&s SA company. Alvarez H.M and Lanfranconi M.P. are career researchers of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11274_2018_2499_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1565 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • O. Marisa Herrero
    • 1
  • María S. Villalba
    • 1
  • Mariana P. Lanfranconi
    • 1
  • Héctor M. Alvarez
    • 1
  1. 1.Instituto de Biociencias de la Patagonia (INBIOP)Universidad Nacional de la Patagonia San Juan Bosco y CONICETComodoro RivadaviaArgentina

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