Molecular cloning, identification of GSTs family in sunflower and their regulatory roles in biotic and abiotic stress
Glutathione-S-transferase (GST) genes exist widely in plants and play major role in metabolic detoxification of exogenous chemical substances and oxidative stress. In this study, 14 sunflower GST genes (HaGSTs) were identified based on the sunflower transcriptome database that we had constructed. Full-length cDNA of 14 HaGTSs were isolated from total RNA by reverse transcription PCR (RT-PCR). Sunflower was received biotic stress (Sclerotinia sclerotiorum) and abiotic stress (NaCl, low-temperature, drought and wound). GST activity was measured by using the universal substrate. The results showed that most of the HaGSTs were up-regulated after NaCl and PEG6000-induced stresses, while a few HaGSTs were up-regulated after S. sclerotiorum, hypothermia and wound-induced stressed, and there was correlation between the changes of GST activity and the expression of HaGSTs, indicating that HaGSTs may play regulatory role in the biotic and abiotic stress responses. 14 HaGSTs from sunflower were identified, and the expression of HaGSTs were tissue-specific and played regulatory roles in both stress and abiotic stress.
KeywordsGlutathione-S-transferase Sunflower HaGSTs Biotic stress Abiotic stress Oxidative stress
Thanks to Dr. Chen Jishan and Dr. Ma Jun from Heilongjiang Academy of Agricultural Sciences for their help in data analysis.
This word sponsored by Project of National Specialty Oil Industry Technology System (Grant Number CARS-14-1-20) and Postdoctoral Research Fund of Heilongjiang Academy of Agricultural Sciences (Grant Number LRB 185216).
Compliance with ethical standards
Conflict of interest
The authors declare there have no conflict of interest.
This study was approved by The Ethics Committee of Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences.
Participants have provided their written informed consent to participate in this study.
- Chronopoulou E, Georgakis N, Nianiou-Obeidat I, Madesis P, Perperopoulou F, Pouliou F, Vasilopoulou E, Ioannou E, Ataya F, Labrou N (2017) Plant glutathione transferases in abiotic stress response and herbicide resistance. In: Glutathione in plant growth, development, and stress tolerance. Springer, Cham, pp. 215–233CrossRefGoogle Scholar
- Csiszár J, Váry Z, Horváth E, Gallé Á, Tari I (2011) Role of glutathione transferases in the improved acclimation to salt stress in salicylic acid-hardened tomato. Acta Biol Szeged 55(1):67–68Google Scholar
- Czarnocka W, Karpiński S (2018) Friend or foe? Reactive oxygen species production, scavenging and signaling in plant response to environmental stresses. Free Radical Biol Med. https://doi.org/10.1016/j.freeradbiomed.2018.01.011 Google Scholar
- Ding N, Wang A, Zhang X, Wu Y, Wang R, Cui H, Huang R, Luo Y (2017) Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST -mediated networks in aboveground and underground tissues in response to abiotic stresses. BMC Plant Biol 17(1):225CrossRefPubMedPubMedCentralGoogle Scholar
- Dixon DP, Lapthorn A, Edwards R (2005) Plant glutathione transferases. Genome Biol 401(3):169–186Google Scholar
- Habig WHJW. (1981) Assay for differentiation of GST. Method Enzymol 77:735–740Google Scholar
- Islam M, Chowdhury A, Rahman M, Rohman M (2015) Comparative investigation of glutathione S-transferase (GST) in different crops and purification of high active GSTs from Onion (Allium cepa L.). J Plant Sci 3:162–170Google Scholar
- Labrou NE, Tsaftaris A, Madesis P, Nianiou-Obeidat I, Axarli I, Bosmali E, Voulgari G, Perperopoulou F, Pouliou F, Chantzikonstantinou M (2015) Plant glutathione transferases: structure, antioxidant catalytic function and in planta protective role in biotic and abiotic stress. Curr Chem Biol 8(2):58–75CrossRefGoogle Scholar
- Li-Gong MA, Meng QL, Zhang YH, Liu ZH, Wang ZY, University NF (2015) Clone and function of a glutathione-S-transferase gene from sunflower (Helianthus annuus). Chin J Oil Crop Sci 37(5):635–643Google Scholar
- Lo CL, Madesis P, Tsaftaris A, Lo Piero AR (2015) Tobacco plants over-expressing the sweet orange tau glutathione transferases (CsGSTUs) acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses. Phytochemistry 116(1):69Google Scholar
- Mayer Z, Duc N, Posta K (2017) Gene expression of glutathione-S-transferase in sunflower (Helianthus annuus L.) inoculated with arbuscular mycorrhizal fungi under temperature stressesGoogle Scholar
- Pierre-Alexandre L, Bastiaan B, Olivier K, Arnaud H, Nicolas R (2014) The still mysterious roles of cysteine-containing glutathione transferases in plants. Front Pharmacol 5(192):1–22Google Scholar
- Sappl PG, Carroll AJ, Clifton R, Lister R, Whelan J, Millar AH, Singh KB (2009) The Arabidopsis glutathione transferase gene family displays complex stress regulation and co-silencing multiple genes results in altered metabolic sensitivity to oxidative stress. Plant J Cell Mol Biol 58(1):53–68CrossRefGoogle Scholar
- Soonyoung A, Seonae K, Yun HK (2016) Glutathione S-transferase genes differently expressed by pathogen-infection in Vitis flexuosa. Plant Breed Biotechnol 4(5):61–70Google Scholar
- Vollenweider S, Weber H, Stolz S, Chételat A, Farmer EE (2010) Fatty acid ketodienes and fatty acid ketotrienes: michael addition acceptors that accumulate in wounded and diseased Arabidopsis leaves. Plant J 24(4):467Google Scholar