Molecular cloning, identification of GSTs family in sunflower and their regulatory roles in biotic and abiotic stress
Glutathione-S-transferase (GST) genes exist widely in plants and play major role in metabolic detoxification of exogenous chemical substances and oxidative stress. In this study, 14 sunflower GST genes (HaGSTs) were identified based on the sunflower transcriptome database that we had constructed. Full-length cDNA of 14 HaGTSs were isolated from total RNA by reverse transcription PCR (RT-PCR). Sunflower was received biotic stress (Sclerotinia sclerotiorum) and abiotic stress (NaCl, low-temperature, drought and wound). GST activity was measured by using the universal substrate. The results showed that most of the HaGSTs were up-regulated after NaCl and PEG6000-induced stresses, while a few HaGSTs were up-regulated after S. sclerotiorum, hypothermia and wound-induced stressed, and there was correlation between the changes of GST activity and the expression of HaGSTs, indicating that HaGSTs may play regulatory role in the biotic and abiotic stress responses. 14 HaGSTs from sunflower were identified, and the expression of HaGSTs were tissue-specific and played regulatory roles in both stress and abiotic stress.
KeywordsGlutathione-S-transferase Sunflower HaGSTs Biotic stress Abiotic stress Oxidative stress
Thanks to Dr. Chen Jishan and Dr. Ma Jun from Heilongjiang Academy of Agricultural Sciences for their help in data analysis.
This word sponsored by Project of National Specialty Oil Industry Technology System (Grant Number CARS-14-1-20) and Postdoctoral Research Fund of Heilongjiang Academy of Agricultural Sciences (Grant Number LRB 185216).
Compliance with ethical standards
Conflict of interest
The authors declare there have no conflict of interest.
This study was approved by The Ethics Committee of Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences.
Participants have provided their written informed consent to participate in this study.
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