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Microbial community and physicochemical dynamics during the production of ‘Chicha’, a traditional beverage of Indigenous people of Brazil

  • Ludmila Vilela Resende
  • Leticia Kleinhans Pinheiro
  • Maria Gabriela da Cruz Pedroso Miguel
  • Cíntia Lacerda Ramos
  • Danielle Marques Vilela
  • Rosane Freitas Schwan
Original Paper

Abstract

The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemically characterized by gas and liquid chromatography (HPLC and GC-MS). Aerobic mesophilic bacteria (AMB) (35.8% of total of isolated microorganisms), lactic acid bacteria (LAB) (21.6%) and yeast (42.6%) were identified. Species of the genera Klebsiella, Bacillus, Staphylococcus, Micrococcus, Enterobacter, and Weissella were identified. Rhodotorula mucilaginosa, Lodderomyces elongisporus, Candida metapsilosis, and C. bohicensis were the yeasts found. The LAB isolates detected were responsible for the high concentrations of lactic acid found during the fermentation process (1.2 g L− 1), which is directly related to the decrease in pH values (from 6.95 to 3.70). Maltose was the main carbohydrate detected during corn fermentation (7.02 g L− 1 with 36 h of fermentation). Ethanol was found in low concentrations (average 0.181 g L− 1), making it possible to characterize the beverage as non-alcoholic. Twelve volatile compounds were identified by gas chromatography; belonging to the groups acids, alcohols aldehydes, acetate and others. MALDI-TOF was successfully used for identification of microbiota. Weissella confusa and W. cibaria were detected in the final product (after 36 h of fermentation), W. confusa is often classified as probiotic and deserve further application studies.

Keywords

Microbial succession MALDI-TOF MS Indigenous fermentation Volatile compounds Corn 

Notes

Funding

The funding was provided by CNPq, FAPEMIG, CAPES.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Ludmila Vilela Resende
    • 1
  • Leticia Kleinhans Pinheiro
    • 1
  • Maria Gabriela da Cruz Pedroso Miguel
    • 2
  • Cíntia Lacerda Ramos
    • 2
  • Danielle Marques Vilela
    • 1
  • Rosane Freitas Schwan
    • 2
  1. 1.Faculty of Biological and Environmental SciencesFederal University of Grande DouradosDouradosBrazil
  2. 2.Department of BiologyFederal University of LavrasLavrasBrazil

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