Subcritical n-hexane/isopropanol extraction of lipid from wet microalgal pastes of Scenedesmus obliquus
- 121 Downloads
Subcritical co-solvents of n-hexane/isopropanol were primarily utilized to extract lipid from wet microalgal pastes of Scenedesmus obliquus. The effects of key operational parameters were investigated, and the optimal parameters were obtained: solvent ratio of n-hexane to isopropanol was 3:2 (V:V), phase ratio of co-solvents to microalgal biomass was 35:1 (mL:g), reactor stirring speed was 900 rpm, extraction time was 60 min. Additional pretreatment with acid, ultrasonic and microwave as well as enhanced subcritical pressure/heating treatments were also applied to further study their effects on lipid extraction. The results showed that the lipid recovery rate with acid pretreatment was 8.6 and 6.2% higher than ultrasonic and microwave pretreatment; the optimum enhanced subcritical condition was 55 °C with atmospheric pressure. Under optimal operating conditions, the lipid and FAME yield were 13.5 and 7.2%, which was 82.6 and 135.1% higher than the traditional method. The results indicated that the subcritical n-hexane/isopropanol extraction process had promising application potential.
KeywordsLipid extraction Subcritical Solvents extraction Wet microalgae pastes Pretreatment
This work was supported by the National Natural Science Foundation of China (No. 50978069) and Shenzhen Science and Technology Innovation Project (JCYJ20150529114024234, JCYJ20170307150223308).
- Chen S, Jiang J, Nie X (2007) Research progress on the low-temperature flow property of biodiesel. Biomass Chem Eng 41(6):42–46Google Scholar
- Guo J, Qiu T, Yang R, Fan X (2007) Progress in subcritical water extraction technique for extraction of natural products. Mod Chem Industry 27(12):19–24Google Scholar
- Jiang X, Zhou H, Jin Q, Wang X (2012) Progress in extraction of microalgae oil. China Oils Fats 37(10):62–66Google Scholar
- Wan C, Huang F, Zhang M, Li W, Huang Q (2014) Subcritical fluid extraction of oil from cold press linseed cake. Trans CSAE 30(21):324–331Google Scholar
- Xia J, Wan M, Wang R, Liu P, Li L, Huang B, Qiu G (2009) Current status and progress of microalgal biodiesel. China Biotechnol 29(7):118–126Google Scholar