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Production of d-alanine from dl-alanine using immobilized cells of Bacillus subtilis HLZ-68

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Abstract

Immobilized cells of Bacillus subtilis HLZ-68 were used to produce d-alanine from dl-alanine by asymmetric degradation. Different compounds such as polyvinyl alcohol and calcium alginate were employed for immobilizing the B. subtilis HLZ-68 cells, and the results showed that cells immobilized using a mixture of these two compounds presented higher l-alanine degradation activity, when compared with free cells. Subsequently, the effects of different concentrations of polyvinyl alcohol and calcium alginate on l-alanine consumption were examined. Maximum l-alanine degradation was exhibited by cells immobilized with 8% (w/v) polyvinyl alcohol and 2% (w/v) calcium alginate. Addition of 400 g of dl-alanine (200 g at the beginning of the reaction and 200 g after 30 h of incubation) into the reaction solution at 30 °C, pH 6.0, aeration of 1.0 vvm, and agitation of 400 rpm resulted in complete l-alanine degradation within 60 h, leaving 185 g of d-alanine in the reaction solution. The immobilized cells were applied for more than 15 cycles of degradation and a maximum utilization rate was achieved at the third cycle. d-alanine was easily extracted from the reaction solution using cation-exchange resin, and the chemical and optical purity of the extracted d-alanine was 99.1 and 99.6%, respectively.

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Acknowledgements

This work was financially support by the Science Research and Technology Development Project of Nanning (20125193) and Wuzou (201201023).

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, Guangxi, China

    Yangyang Zhang, Xiangping Li, Caifei Zhang, Xiaodong Yu, Shihai Huang, Lianwei Li & Shiyu Liu

  2. High-tech Industrial Development Zone, Xixiangtang, Nanning, 530004, Guangxi, China

    Fei Huang

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  1. Yangyang Zhang
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  2. Xiangping Li
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  3. Caifei Zhang
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  4. Xiaodong Yu
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  5. Fei Huang
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  6. Shihai Huang
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  7. Lianwei Li
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  8. Shiyu Liu
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Correspondence to Shihai Huang.

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Zhang, Y., Li, X., Zhang, C. et al. Production of d-alanine from dl-alanine using immobilized cells of Bacillus subtilis HLZ-68. World J Microbiol Biotechnol 33, 176 (2017). https://doi.org/10.1007/s11274-017-2341-3

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  • DOI: https://doi.org/10.1007/s11274-017-2341-3

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