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Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus

  • El Hadj Driche
  • Nasserdine Sabaou
  • Christian Bijani
  • Abdelghani Zitouni
  • Frédéric Pont
  • Florence Mathieu
  • Boubekeur Badji
Original Paper

Abstract

A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368T with 99.6% of similarity. However, the comparison of the morphological and the physiological characteristics of the strain with those of the nearest species showed significant differences. The strain AT37 secreted the antibiotic optimally during mid-stationary phase of growth. One active compound (AT37-1) was extracted from the culture broth with dichloromethane, separated on silica gel plates and purified by HPLC. Based on spectroscopic analysis of UV–Visible, infrared, and 1H and 13C NMR spectra and spectrometric analysis, the chemical structure of the compound AT37-1 was identified as 5-[(5E,7E,11E)-2,10-dihydroxy-9,11-dimethyl-5,7,11-tridecatrien-1-yl]-2-hydroxy-2-(1-hydroxyethyl)-4-methyl-3(2H)-furanone. Minimum inhibitory concentrations and minimum biofilm inhibitory concentration (MBIC50) of this compound showed significant activity against multidrug-resistant S. aureus with 15–30 and 10–15 μg/mL, respectively.

Keywords

Streptomyces Saharan soil Furanone derivative Antibiofilm Multidrug-resistant Staphylococcus aureus 

Notes

Acknowledgements

This work was gratefully supported by the «Comité mixte Franco-Algérien de l’Accord Programme TASSILI (PHC No. 15 MDU 932; Campus France: 33000WC)».

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

Supplementary material

11274_2017_2265_MOESM1_ESM.doc (80 kb)
Fig. S1 HPLC purification process for the active compound secreted by Streptomyces sp. AT37. (a) First HPLC injection of the active fraction ATa (Rf = 0.8 in ethyl-acetate–methanol, 100–15 v/v) resulting from the TLC silica gel. (b) Final purification of the active compound AT37-1 after re-injection. Column, ZORBAX SD C18; continuous linear gradient system, 10–100 % methanol in water; flow rate, 1.2 mL/min; detection, 220 nm (DOC 79 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • El Hadj Driche
    • 1
    • 2
  • Nasserdine Sabaou
    • 1
  • Christian Bijani
    • 3
  • Abdelghani Zitouni
    • 1
  • Frédéric Pont
    • 4
  • Florence Mathieu
    • 5
  • Boubekeur Badji
    • 1
  1. 1.Laboratoire de Biologie des Systèmes Microbiens (LBSM)Ecole Normale SupérieureKoubaAlgeria
  2. 2.Département de Biologie, Faculté des Sciences de la Nature et de la VieUniversité Hassiba Benbouali de ChlefChlefAlgeria
  3. 3.Laboratoire de chimie de coordination (LCC), CNRSUniversité de Toulouse, UPS, INPT, LCCToulouseFrance
  4. 4.Proteomics Group, INSERM UMR1037Centre de Recherches en Cancérologie de Toulouse (CRCT)ToulouseFrance
  5. 5.Laboratoire de Génie Chimique, LGC, UMR 5503 (CNRS/INPT/UPS)Université de ToulouseToulouseFrance

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