Possible correlation between levansucrase production and probiotic activity of Bacillus sp. isolated from honey and honey bee
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Five bacterial isolates from honey and bee gut were selected based on their high levansucrase activity and levan yield which were strongly positively correlated. All isolates showed good tolerance to temperature up to 70 °C, to NaCl up to 3 M and to 0.1% H2O2. They maintained over 59 and 64% survival at pH 9.0 and 2.0 respectively, but showed varying tolerance to 0.1% bile salts and pancreatic enzymes. Most isolates were susceptible to widely used antibiotics, but demonstrated diverse antimicrobial activity. Non hemolytic isolates were identified on the basis of 16S rRNA sequencing as Bacillus subtilis HMNig-2 and B. subtilis MENO2 with 97% homology. They exhibited promising probiotic characteristics and achieved highest levansucrase activity of 94.1 and 81.5 U/mL respectively. Both exhibited highest biofilm formation ability in static microtiter plate assay. Also, they achieved 34 and 26% adhesion respectively to Caco-2cells and had highest free radical scavenging activity of 30.8 and 26.2% respectively. The levans of the two isolates showed good antimicrobial activity against some pathogens and exhibited positive prebiotic effect (prebiotic index >1) with Lactobacillus casei and Lactobacillus reuteri. Results suggest a correlation between levansucrase production, levan yield and pre-probiotic activities of the studied strains.
KeywordsHoney isolates Probiotic bacteria Levansucrase Levan Prebiotic
This work was supported by National Research Center, Chemistry of Natural and Microbial Products Department by project Grant Number 10050305. Special thanks to Prof. Harold Corke (Shanghai Jiao Tong University, China) for his generous help in revising the paper’s language.
- Brown SM, Howell ML, Vasil ML, Anderson AJ, Hassett DJ (1995) Cloning and characterization of the KatB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-inducible catalase, purification of KatB, cellular localization and demonstration that it is essential for optimal resistance to hydrogen peroxide. J Bacteriol 177:6536–6544CrossRefGoogle Scholar
- Chaiyawan N, Taveeteptaiku P, Wannissorn B, Itsaranuwat P (2010) Characterization and probiotic properties of Bacillus strains isolated frombroiler. Thai J Vet Med 40:207–2014Google Scholar
- Clinical and Laboratory Standards Institute (2014) Performance standards for antimicrobial susceptibility testing; 24th informational supplement CLSI M100-S24. Clinical and Laboratory Standards Institute, WayneGoogle Scholar
- Coconnier MH, Klaenhammer TR, Kerneis S (1992) Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl Environ Micro 58:2034–2039Google Scholar
- Dal Bello F, Walter J, Hertel C, Hammes WP (2001) In vitro study of prebiotic properties of levan-type exopolysaccharides from Lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis. Syst Appl Microbiol 24:232–237. doi: 10.1078/0723-2020-00033 CrossRefGoogle Scholar
- Esawy MA, Awad GEA, Ahmed EF, Danial EN, Mansour NM (2012a) Evaluation of honey as new reservoir for probiotic bacteria. Adv Food Sci 34:72–81Google Scholar
- FAO/WHO (2001) Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a Joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria 1–4, Oct. American Córdoba Park Hotel, Córdoba, p 6Google Scholar
- Harely JP, Prescott L (1996) Laboratoryexercises in microbiology. McGraw Hill, New YorkGoogle Scholar
- Hussein MM, Kheiralla ZM, Helal MM, Saker EA (2010) The prebiotic activities of oligosaccharides derived from partial hydrolysis of commercial Algal polysaccharides. Egypt Pharm 9:1–23Google Scholar
- Manhar AK, Bashir Y, Saikia D, Nath D, Gupta K, Konwar BK, Kumar R, Namsa ND, Mandal M (2016) Cellulolytic potential of probiotic Bacillus subtilis AMS6 isolated from traditional fermented soybean (Churpi): an in vitro study with regards to application as an animal feed additive. Microbiol Res 186:62–70. doi: 10.1016/j.micres.2016.03.004 CrossRefGoogle Scholar
- Takahashi N, Xiao J-Z, Miyaji K, Yaeshiima T, Hiramatsu A, Iwatsuki K, Kokubo S, Hosono A (2004) Selection of acid tolerant bifidobacteria and evidence for a lowpH-inducible acid tolerance response in Bifidobacterium longum. J Dairy Res 71:340–345. doi: 10.1017/S0022029904000251 CrossRefGoogle Scholar