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The yeast Scheffersomyces amazonensis is an efficient xylitol producer

Abstract

This study assessed the efficiency of Scheffersomyces amazonensis UFMG-CM-Y493T, cultured in xylose-supplemented medium (YPX) and rice hull hydrolysate (RHH), to convert xylose to xylitol under moderate and severe oxygen limitation. The highest xylitol yields of 0.75 and 1.04 g g−1 in YPX and RHH, respectively, were obtained under severe oxygen limitation. However, volumetric productivity in RHH was ninefold decrease than that in YPX medium. The xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in the YPX cultures were strictly dependent on NADPH and NAD+ respectively, and were approximately 10% higher under severe oxygen limitation than under moderate oxygen limitation. This higher xylitol production observed under severe oxygen limitation can be attributed to the higher XR activity and shortage of the NAD+ needed by XDH. These results suggest that Sc. amazonensis UFMG-CM-Y493T is one of the greatest xylitol producers described to date and reveal its potential use in the biotechnological production of xylitol.

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Acknowledgements

RMC acknowledge the financial support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, PDEE, Process No. 4782-11-9). This work was co-funded by the European Commission in the framework of EU-Brazil Project ProEthanol2G ‘‘Integration of Biology and Engineering into an Economical and Energy-Efficient 2G Bioethanol Biorefinery’’ (FP7-251151), by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Process Nos. 551392/2010-0, 551245/2010-7 and 560715/2010-2), by the Financiadora de Estudos e Projetos (FINEP, Process No. 2084/07) and by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG).

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Correspondence to Carlos A. Rosa.

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The authors declare that they have no conflict of interest.

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Cadete, R.M., Melo-Cheab, M.A., Viana, A.L. et al. The yeast Scheffersomyces amazonensis is an efficient xylitol producer. World J Microbiol Biotechnol 32, 207 (2016). https://doi.org/10.1007/s11274-016-2166-5

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Keywords

  • Bioconversion
  • Hemicellulose hydrolysates
  • Scheffersomyces amazonensis
  • Xylitol
  • d-Xylose