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The combination of osthole with baicalin protects mice from Staphylococcus aureus pneumonia

  • Shui Liu
  • Bowen Liu
  • Zhao-Qing Luo
  • Jiaming Qiu
  • Xuan Zhou
  • Gen Li
  • Bing Zhang
  • Xuming Deng
  • Zhenguo YangEmail author
  • Jianfeng WangEmail author
Short Communication

Abstract

We reported the inhibition of α-Hemolysin (Hla) production in methicillin-resistant Staphylococcus aureus USA300 by osthole and further investigated the combination of osthole and baicalin in the treatment of staphylococcal pneumonia. Using cytotoxicity assays and a mouse model of intranasal lung infection, we evaluated the effect of combined therapy. Our results suggest that the combination of osthole and baicalin alleviated S. aureus-mediated A549 cell injury and protected mice from S. aureus pneumonia.

Keywords

Staphylococcus aureus Osthole Baicalin Alpha-hemolysin 

Notes

Acknowledgments

This work was supported by the National Basic Research Program of China (Grant 2013CB127205), the National Nature Science Foundation of China (Grant 31130053) and the National 863 program (Grant 2012AA020303).

Compliance with ethical standards

Conflicts of interest

The authors declare no conflict of interest.

Ethical standards

The animal studies were performed according to the Regulations for the Administration of Affairs Concerning Experimental Animals (1988.11), and all experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Jilin University.

References

  1. Bartlett AH, Foster TJ, Hayashida A, Park PW (2008) Alpha-toxin facilitates the generation of CXC chemokine gradients and stimulates neutrophil homing in Staphylococcus aureus pneumonia. J Infect Dis 198:1529–1535. doi: 10.1086/592758 CrossRefGoogle Scholar
  2. Bubeck Wardenburg J, Schneewind O (2008) Vaccine protection against Staphylococcus aureus pneumonia. J Exp Med 205:287–294. doi: 10.1084/jem.20072208 CrossRefGoogle Scholar
  3. Bubeck Wardenburg J, Patel RJ, Schneewind O (2007) Surface proteins and exotoxins are required for the pathogenesis of Staphylococcus aureus pneumonia. Infect Immun 75:1040–1044. doi: 10.1128/IAI.01313-06 CrossRefGoogle Scholar
  4. Cegelski L, Marshall GR, Eldridge GR, Hultgren SJ (2008) The biology and future prospects of antivirulence therapies. Nat Rev Microbiol 6:17–27. doi: 10.1038/nrmicro1818 CrossRefGoogle Scholar
  5. Dong J et al (2013) Apigenin alleviates the symptoms of Staphylococcus aureus pneumonia by inhibiting the production of alpha-hemolysin. FEMS Microbiol Lett 338:124–131. doi: 10.1111/1574-6968.12040 CrossRefGoogle Scholar
  6. Grundmann H, Aires-de-Sousa M, Boyce J, Tiemersma E (2006) Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368:874–885. doi: 10.1016/S0140-6736(06)68853-3 CrossRefGoogle Scholar
  7. Katragkou A et al (2015) In vitro interactions between farnesol and fluconazole, amphotericin B or micafungin against Candida albicans biofilms. J Antimicrob Chemother 70:470–478. doi: 10.1093/jac/dku374 CrossRefGoogle Scholar
  8. Lackner M, Fernandez-Silva F, Guarro J, Lass-Florl C (2014) Assessing micafungin/triazole combinations for the treatment of invasive scedosporiosis due to Scedosporium apiospermum and Scedosporium boydii. J Antimicrob Chemother 69:3027–3032. doi: 10.1093/jac/dku224 CrossRefGoogle Scholar
  9. Liu S et al (2015) Diosmetin inhibits the expression of alpha-hemolysin in Staphylococcus aureus. Antonie Van Leeuwenhoek 108:383–389. doi: 10.1007/s10482-015-0491-6 CrossRefGoogle Scholar
  10. Lowy FD (1998) Staphylococcus aureus infections. N Engl J Med 339:520–532. doi: 10.1056/NEJM199808203390806 CrossRefGoogle Scholar
  11. Powers JH (2004) Antimicrobial drug development–the past, the present, and the future. Clin Microbiol Infect 10(Suppl 4):23–31. doi: 10.1111/j.1465-0691.2004.1007.x CrossRefGoogle Scholar
  12. Qiu J et al (2012) Baicalin protects mice from Staphylococcus aureus pneumonia via inhibition of the cytolytic activity of alpha-hemolysin. J Infect Dis 206:292–301. doi: 10.1093/infdis/jis336 CrossRefGoogle Scholar
  13. Rasko DA, Sperandio V (2010) Anti-virulence strategies to combat bacteria-mediated disease. Nat Rev Drug Discov 9:117–128. doi: 10.1038/nrd3013 CrossRefGoogle Scholar
  14. Rossolini GM, Arena F, Pecile P, Pollini S (2014) Update on the antibiotic resistance crisis. Curr Opin Pharmacol 18:56–60. doi: 10.1016/j.coph.2014.09.006 CrossRefGoogle Scholar
  15. Wang J et al (2015) Morin hydrate attenuates Staphylococcus aureus virulence by inhibiting the self-assembly of alpha-hemolysin. J Appl Microbiol 118:753–763. doi: 10.1111/jam.12743 CrossRefGoogle Scholar
  16. Xiong YQ, Willard J, Yeaman MR, Cheung AL, Bayer AS (2006) Regulation of Staphylococcus aureus alpha-toxin gene (hla) expression by agr, sarA, and sae in vitro and in experimental infective endocarditis. J Infect Dis 194:1267–1275. doi: 10.1086/508210 CrossRefGoogle Scholar
  17. Yang SM et al (2014) Osthole improves an accelerated focal segmental glomerulosclerosis model in the early stage by activating the Nrf2 antioxidant pathway and subsequently inhibiting NF-kappaB-mediated COX-2 expression and apoptosis. Free Radic Biol Med 73:260–269. doi: 10.1016/j.freeradbiomed.2014.05.009 CrossRefGoogle Scholar
  18. Zhou X et al (2015) Phloretin derived from apple can reduce alpha-hemolysin expression in methicillin-resistant Staphylococcus aureus USA300. World J Microbiol Biotechnol 31:1259–1265. doi: 10.1007/s11274-015-1879-1 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Shui Liu
    • 1
  • Bowen Liu
    • 1
  • Zhao-Qing Luo
    • 2
  • Jiaming Qiu
    • 1
  • Xuan Zhou
    • 1
  • Gen Li
    • 1
  • Bing Zhang
    • 1
  • Xuming Deng
    • 1
  • Zhenguo Yang
    • 1
    Email author
  • Jianfeng Wang
    • 1
    Email author
  1. 1.Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary MedicineJilin UniversityChangchunChina
  2. 2.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

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