Bacillus subtilis based-formulation for the control of postbloom fruit drop of citrus

  • Mariana Nadjara Klein
  • Aline Caroline da Silva
  • Katia Cristina KupperEmail author
Original Paper


Postbloom fruit drop (PFD) caused by Colletotrichum acutatum affects flowers and causes early fruit drop in all commercial varieties of citrus. Biological control with the isolate ACB-69 of Bacillus subtilis has been considered as a potential method for controlling this disease. This study aimed to develop and optimize a B. subtilis based-formulation with a potential for large-scale applications and evaluate its effect on C. acutatum in vitro and in vivo. Bacillus subtilis based-formulations were developed using different carrier materials, and their ability to control PFD was evaluated. The results of the assays led to the selection of the B. subtilis based-formulation with talc + urea (0.02 %) and talc + ammonium molybdate (1 mM), which inhibited mycelial growth and germination of C. acutatum. Studies with detached citrus flowers showed that the formulations were effective in controlling the pathogen. In field conditions, talc + urea (0.02 %) provided 73 % asymptomatic citrus flowers and 56 % of the average number of effective fruit (ANEF), equating with fungicide treatment. On the contrary, non-treated trees had 8.8 % of asymptomatic citrus flowers and 0.83 % ANEF. The results suggest that B. subtilis based-formulations with talc as the carrier supplemented with a nitrogen source had a high potential for PFD control.


Additives Biological control Bioproducts Colletotrichum acutatum Survival 



We thank the National Council for Scientific and Technological Development—CNPq—(Proceeding No. 471065/2011-0 granted to Katia C. Kupper) for the financial support and FAPESP (The State of São Paulo Research Foundation) for the scholarship granted to Mariana N. Klein (Proceeding No. 2009/12854).


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Mariana Nadjara Klein
    • 1
  • Aline Caroline da Silva
    • 1
  • Katia Cristina Kupper
    • 2
    Email author
  1. 1.Agroecology and Rural Development Program, Postgraduate SchoolUniversidade Federal de São CarlosArarasBrazil
  2. 2.Laboratory of Plant Pathology and Biological ControlCentro de Citricultura “Sylvio Moreira”/IACCordeirópolisBrazil

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