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Optimization of substrate preparation for oyster mushroom (Pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases I and II)

  • Fabrício Rocha VieiraEmail author
  • Meire Cristina Nogueira de Andrade
Original Paper

Abstract

In recent years, oyster mushroom (Pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in Brazil. Among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. Looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbens grass, brizantha grass and sugarcane straw) and nitrogen supplementation (with or without wheat bran) on oyster mushroom yield and biological efficiency (BE). The substrate composted for 7 days with conditioning showed higher yield and biological efficiency of mushroom (24.04 and 100.54 %, respectively). Substrates without conditioning (7 and 14 days of composting) showed smaller mushroom yield and biological efficiency. Among the raw materials tested, brizantha grass showed higher mushroom yield followed by decumbens grass, sugarcane straw and wheat straw (28.5, 24.32, 23.5 and 19.27 %, respectively). Brizantha grass also showed higher biological efficiency followed by sugarcane straw, decumbens grass and wheat straw (123.95, 103.70, 96.90 and 86.44 %, respectively). Supplementation with wheat bran improved yield and biological efficiency in all substrate formulations tested; thus, oyster mushroom yield and biological efficiency were influenced by substrate formulation (raw materials), supplementation and composting conditions.

Keywords

Composting Pleurotus ostreatus Yield Supplement Raw materials 

Notes

Acknowledgments

This research was supported by Coordination for the Improvement of Higher Level Personnel (CAPES Foundation), No. 005707/2012-05. We are grateful to Graduate Program (Energy in Agriculture) of College of Agronomic Sciences, Sao Paulo State University, for constantly financial support.

References

  1. Bertoldi M, Vallini G, Pera A (1983) The biology of composting: a review. Wast Manag Res 1(2):157–176CrossRefGoogle Scholar
  2. Bonatti M, Karnopp P, Soares HM, Furlan SA (2004) Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food Chem 88(3):425–428CrossRefGoogle Scholar
  3. Brasil (2007) Ministério da Agricultura, Pecuária e Abastecimento – Manual de métodos analíticos oficiais para fertilizantes minerais, orgânicos, organominerais e corretivos. http://www.agricultura.pr.gov.br/arquivos/File/PDF/in_28_07_anexo.pdf. Accessed 25 May 2013
  4. Chang ST, Miles PG (2004) Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact. CRC Press, Boca RatonCrossRefGoogle Scholar
  5. Choi KW (2007) Shelf cultivation of oyster mushroom. In: MushWorld (ed) Mushroom growers’ handbook 1. Oyster mushroom cultivation, MushWorld—Heineart Inc, SeoulGoogle Scholar
  6. Curvetto NR, Figlas D, Devalis R, Delmastro S (2002) Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N-NH4 + and/or MN (II). Bioresour Technol 84(2):171–176CrossRefGoogle Scholar
  7. Hernández D, Sánchez JE, Yamasaki K (2003) A simple procedure for preparing substrate for Pleurotus ostreatus cultivation. Bioresour Technol 90:145–150CrossRefGoogle Scholar
  8. Johnson RR, Balwani TL, Johnson LJ, McClume KE, Dehority BA (1966) corn plant maturity II. Effect on In vitro cellulose digestibility and soluble carbohydrate content. J Anim Sci 25:617–623CrossRefGoogle Scholar
  9. Lee HY, Won-Rok K, Min BH (2002) Automation of solid-state bioreactor for Oyster Mushroom composting. Microbiol 30:228–232Google Scholar
  10. Lyons GA, Sharma HSS, Kilpatrick M, Cheung L, Moore S (2006) Monitoring of changes in substrate characteristics during mushroom compost production. J Agric Food Chem 54:4658–4667CrossRefGoogle Scholar
  11. Nunes MD, Da Luz JMR, Paes SA, Ribeiro JJO, Silva MCS, Kasuya MCM (2012) Nitrogen supplementation on the productivity and the chemical composition of oyster mushroom. J food Res 1(2):113–119CrossRefGoogle Scholar
  12. Obodai M, Cleland-Okine J, Vowotor KA (2003) Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. J Ind Microbiol Biotechnol 30:146–149CrossRefGoogle Scholar
  13. Oei P (2003) Mushroom cultivation. Backhuys Publishers, LeidenGoogle Scholar
  14. Philippoussis A, Zervakis G, Diamantopoulou P (2001) Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J Microbiol Biotechnol 17:191–200CrossRefGoogle Scholar
  15. Rizki M, Tamai Y (2011) Effects of different nitrogen rich substrates and their combination to the yield performance of oyster mushroom (Pleurotus ostreatus). World J Microbiol Biotechnol 27:1695–1702CrossRefGoogle Scholar
  16. Royse DJ (2013) Trends in Mushroom production worldwide. In: Sales-Campos C, Abreu RLS, Vianez BF, Urben AF (eds) Anais do VII Simpósio internacional sobre cogumelos no Brasil, 6th edn. Embrapa, Brasilia, pp 38–47Google Scholar
  17. Royse DJ, Schisler LC (1987) Yield and size of Pleurotus ostreatus and Pleurotus sajor- caju as effected by delayed-release nutrient. Appl Microbiol Biotechnol 26(2):191–194CrossRefGoogle Scholar
  18. Royse DJ, Rhodes TW, Ohga S, Sanchez JE (2004) Yield, mushroom size and time to production of Pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Bioresour Technol 91(1):85–91CrossRefGoogle Scholar
  19. Ryckeboer J, Mergaert J, Vaes K, Klammer S, De Clercq D, Coosemans J, Insam H, Swings J (2003) A survey of bacteria and fungi occurring during composting and self-heating processes. Ann Microbiol 53(4):349–410Google Scholar
  20. Sánchez C (2010) Cultivation of Pleurotus ostreatus and other edible mushroom. Appl Microbiol Biotechnol 80:1321–1337CrossRefGoogle Scholar
  21. Santos FA, Queiróz JH, Colodette JL, Fernandes SA, Guimarãrs VM, Rezende ST (2012) Potential of sugarcane straw for ethanol production. Quim Nova 35(5):1004–1010CrossRefGoogle Scholar
  22. Sharma HSS, Lyons G, Chambers J (2000) Comparison of the changes in mushroom (Agaricus bisporus) compost during windrow and bunker stages of phase I and II. Ann Appl Biol 136(1):59–68CrossRefGoogle Scholar
  23. Sinden JW, Hauser E (1950) The short method of composting. Mushroom Sci 1:52–59Google Scholar
  24. Straatsma G, Samson RA, Olijnsma TW, Op Den Camp HJM, Gerrits JPG, Van Griensven LJLD (1994) Ecology of thermophilic fungi in mushroom compost, with emphasis on Scytalidium thermophilum and growth stimulation of Agaricus bisporus mycelium. Appl Environ Microbiol 60(2):454–458Google Scholar
  25. Straatsma G, Gerrits JPG, Thissen JTNM, Amsing JGM, Loeffen H, Van Griensven LJLD (2000) Adjustment of the composting process for mushroom cultivation based on initial substrate composition. Bioresour Technol 72(1):67–74CrossRefGoogle Scholar
  26. Thambirajah JJ, Zulkali MD, Hashim MD (1995) Microbiological and biochemical changes during the composting of oil palm empty-fruit-bunches. Effect of nitrogen supplementation on the substrate. Bioresour Technol 52(2):133–144CrossRefGoogle Scholar
  27. Tuomela M, Vikman M, Hatakka A, Itavaara M (2000) Biodegradation of lignin in a compost environment: a review. Bioresour Technol 72(2):169–183CrossRefGoogle Scholar
  28. Vajna B, Nagy A, Sajben E (2010) Microbial community structure changes during oyster mushroom substrate preparation. Appl Microbiol Biotechnol 86:367–375CrossRefGoogle Scholar
  29. Van Griensven LJLD (1988) The cultivation of mushrooms. Rustington, SussexGoogle Scholar
  30. Vieira FR, Pereira DM, Andrade MCN, Minhoni MTA (2012) Molecular characterization of Pleurotus ostreatus commercial strains by random amplified polymorphic DNA (RAPD). Afr J Agric Res 8(24):3146–3150Google Scholar
  31. Zervakis GI, Koutrotsios G, Katsaris P (2013) Composted versus raw olive mill waste as substrate for the production of medicinal mushrooms: an assessment of selected cultivation and quality parameters. Biomed Res Int. doi: 10.1155/2013/546830 Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Plant Production, College of Agricultural SciencesSao Paulo State UniversityBotucatuBrazil
  2. 2.Mushroom Research CenterThe Pennsylvania State UniversityUniversity ParkUSA

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